Tazi Neftaha, Pigeon Xavier, Mbuyi-Boisvert Jérôme Mulamba, Giret Simon, Béland François, Rouabhia Mahmoud
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, QC G1V 0A6, Canada.
SiliCycle® Inc. 2500, Parc-Technologique Blvd, Québec, QC G1P 4S6, Canada.
Microorganisms. 2021 Nov 13;9(11):2348. doi: 10.3390/microorganisms9112348.
The most common use of cannabis is smoking. The oral ecosystem, among other constituents, can be deregulated by the presence of cannabis smoke in the oral cavity. We evaluated the effect of cannabis smoke condensate (CSC) on the behavior of , a common yeast found in the oral cavity. The yeast was first cultured with different concentrations of CSC, and its growth was evaluated. The transition from the blastospore to the hyphal form and the hyphae size were assessed after 3 and 6 h, along with biofilm formation after 72 h of contact with CSC. The response of to oxidative (HO) stress was also examined. Our results show that CSC contained high amounts of THC (about 1055 ppm), CBN (63 ppm), and CBG (about 47 ppm). The presence of various concentrations of CSC in the culture medium increased growth. CSC also contributed to increases in both the hyphal length and biofilm mass. Following oxidative stress (HO at either 100 or 500 μM), CSC prevented the damaging effect of HO on both shape and growth. These findings support clinical observations demonstrating that cannabis may promote growth and oral candidiasis.
大麻最常见的使用方式是吸烟。口腔生态系统，除其他成分外，可因口腔中存在大麻烟雾而失调。我们评估了大麻烟雾冷凝物（CSC）对口腔中常见酵母——白色念珠菌行为的影响。首先将酵母与不同浓度的CSC一起培养，并评估其生长情况。在3小时和6小时后评估从芽生孢子到菌丝形态的转变以及菌丝大小，同时评估与CSC接触72小时后的生物膜形成情况。还检测了白色念珠菌对氧化（H₂O₂）应激的反应。我们的结果表明，CSC含有大量的四氢大麻酚（约1055 ppm）、大麻酚（63 ppm）和大麻二酚（约47 ppm）。培养基中不同浓度CSC的存在促进了白色念珠菌的生长。CSC还导致菌丝长度和生物膜质量增加。在氧化应激（100或500 μM的H₂O₂）后，CSC可防止H₂O₂对白色念珠菌形态和生长的破坏作用。这些发现支持了临床观察结果，即大麻可能促进白色念珠菌生长和口腔念珠菌病。
