Duan Zedong, Liao Li, Lai Tingyi, Yang Ruyi, Zhang Jin, Chen Bo
Key Laboratory of Polar Ecosystem and Climate Change, Ministry of Education; Shanghai Key Laboratory of Polar Life and Environment Sciences; and School of Oceanography, Shanghai Jiao Tong University, Shanghai, China.
Key Laboratory for Polar Science, Ministry of Natural Resources, Polar Research Institute of China, Shanghai, China.
Commun Biol. 2025 Mar 5;8(1):369. doi: 10.1038/s42003-025-07780-y.
The Csr (Carbon Storage Regulator) system is pivotal in controlling various cellular functions in most bacteria, primarily through the CsrA protein and its antagonistic sRNAs. However, riboregulatory networks are less explored in non-model organisms, particularly those in extreme environments. In this study, we discovered two new sRNAs of the Csr system, Pf2 and Pf3, in the Arctic bacterium Pseudoalteromonas fuliginea BSW20308, along with the previously known Pf1. By studying the impact of these Pf sRNAs on CsrA targetomes and physiological processes, we found a significant influence on various cellular functions and a collective effect on the interaction dynamics between CsrA and RNAs. Furthermore, we identified additional sRNAs that can interact with CsrA and mRNAs. Overall, our results emphasize the growing influence of the Csr system on cellular physiology through intricate sRNA regulation of CsrA, revealing riboregulatory network complexity and significance in non-model organisms.
碳储存调控（Csr）系统在控制大多数细菌的各种细胞功能中起着关键作用，主要是通过CsrA蛋白及其拮抗sRNA来实现。然而，在非模式生物中，尤其是那些处于极端环境中的生物，核糖调控网络的研究较少。在本研究中，我们在北极细菌暗黑假交替单胞菌BSW20308中发现了Csr系统的两个新sRNA，即Pf2和Pf3，以及之前已知的Pf1。通过研究这些Pf sRNA对CsrA靶标组和生理过程的影响，我们发现它们对各种细胞功能有显著影响，并且对CsrA与RNA之间的相互作用动态有综合效应。此外，我们还鉴定出了其他能够与CsrA和mRNA相互作用的sRNA。总体而言，我们的结果强调了Csr系统通过对CsrA进行复杂的sRNA调控，对细胞生理学的影响日益增大，揭示了非模式生物中核糖调控网络的复杂性和重要性。
