Mohammed Asik Rajmohamed, Manikkaraja Chidhambaram, Tamil Surya Karuppusamy, Suganthy Natarajan, Priya Aarthy Archunan, Mathe Domokos, Sivakumar Muthusamy, Archunan Govindaraju, Padmanabhan Parasuraman, Gulyas Balazs
Department of Animal Science, Bharathidasan University, Tiruchirappalli 620024, India.
Department of Nanoscience and Technology, Alagappa University, Karaikudi 630003, India.
Nanomaterials (Basel). 2021 Nov 22;11(11):3154. doi: 10.3390/nano11113154.
This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of -20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy.
本研究报告了使用氨基酸L-组氨酸作为还原和封端剂合成银纳米颗粒的方法，这是一种环保方法。通过光谱和显微镜研究对制备的L-组氨酸封端银纳米颗粒（L-HAgNPs）进行了表征。合成的球形L-HAgNPs粒径为47.43±19.83 nm，zeta电位为-20.5±0.95 mV。L-HAgNPs的抗癌潜力结果显示，其对SiHa细胞具有剂量依赖性的抗增殖作用，IC值为18.25±0.36 µg/mL。荧光显微镜分析显示，L-HAgNPs诱导活性氧（ROS）介导的线粒体功能障碍，导致凋亡途径激活和DNA损伤，最终导致细胞死亡。总之，L-HAgNPs有望成为宫颈癌治疗的候选药物。
