Department of Pediatrics, Faculty of Health Sciences, McMaster University Medical Centre (MUMC), 1200 Main St. W, Hamilton, ON L8N 3Z5, Canada.
Exerkine Corporation, McMaster University Medical Centre (MUMC), 1200 Main St. W, Hamilton, ON L8N 3Z5, Canada.
Nutrients. 2021 Oct 22;13(11):3726. doi: 10.3390/nu13113726.
We investigated the effects of a novel multi-ingredient supplement comprised of polyphenol antioxidants and compounds known to facilitate mitochondrial function and metabolic enhancement (ME) in a mouse model of obesity. In this study, 6-week-old male C57/BL6J mice were placed on a high-fat diet (HFD; ~60% fat) for 6 weeks, with subsequent allocation into experimentalgroups for 4 weeks: HFD control, HFD + ME10 (10 components), HFD + ME7 (7 components), HFD + ME10 + EX, HFD + EX (where '+EX' animals exercised 3 days/week), and chow-fed control. After the intervention, HFD control animals had significantly greater body weight and fat mass. Despite the continuation of HFD, animals supplemented with multi-ingredient ME or who performed exercise training showed an attenuation of fat mass and preservation of lean body mass, which was further enhanced when combined (ME+EX). ME supplementation stimulated the upregulation of white and brown adipose tissue mRNA transcripts associated with mitochondrial biogenesis, browning, fatty acid transport, and fat metabolism. In WAT depots, this was mirrored by mitochodrial oxidative phosphorylation (OXPHOS) protein expression, and increased in vivo fat oxidation measured via CLAMS. ME supplementation also decreased systemic and local inflammation markers. Herein, we demonstrated that novel multi-ingredient nutritional supplements induced significant fat loss independent of physical activity while preserving muscle mass in obese mice. Mechanistically, these MEs appear to act by inducing a browning program in white adipose tissue and decreasing other pathophysiological impairments associated with obesity, including mitochondrial respiration alterations induced by HFD.
我们研究了一种新型多成分补充剂对肥胖症小鼠模型中多酚抗氧化剂和已知促进线粒体功能和代谢增强 (ME) 的化合物的影响。在这项研究中，将 6 周龄雄性 C57/BL6J 小鼠置于高脂肪饮食 (HFD；~60%脂肪) 中 6 周，随后将其分配到实验组中 4 周：HFD 对照组、HFD+ME10(10 种成分)、HFD+ME7(7 种成分)、HFD+ME10+EX、HFD+EX(其中' + EX '动物每周运动 3 天)和标准饮食对照组。干预后，HFD 对照组动物体重和脂肪量显著增加。尽管继续给予 HFD，但补充多成分 ME 或进行运动训练的动物显示出脂肪量减少和瘦体重保留，当两者结合时(ME+EX)效果进一步增强。ME 补充刺激与线粒体生物发生、褐变、脂肪酸转运和脂肪代谢相关的白色和棕色脂肪组织 mRNA 转录物的上调。在 WAT 中，这与线粒体氧化磷酸化 (OXPHOS) 蛋白表达相匹配，并通过 CLAMS 测量增加体内脂肪氧化。ME 补充还降低了全身和局部炎症标志物。在此，我们证明了新型多成分营养补充剂可在不进行体育活动的情况下诱导肥胖小鼠显著减脂，同时保留肌肉质量。从机制上讲，这些 ME 似乎通过诱导白色脂肪组织中的褐变程序并减少与肥胖相关的其他病理生理损伤来发挥作用，包括 HFD 引起的线粒体呼吸改变。
