College of Food Science and Engineering, Ocean University of China, No.5, Yushan Road, Qingdao, Shandong Province 266003, PR China.
College of Food Science and Engineering, Ocean University of China, No.5, Yushan Road, Qingdao, Shandong Province 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong Province 266237, PR China.
Colloids Surf B Biointerfaces. 2022 Feb;210:112238. doi: 10.1016/j.colsurfb.2021.112238. Epub 2021 Nov 20.
Aquatic collagens, as the alternative sources of mammalian collagen, have received increasing attention due to its low-cost, low-antigenicity, biocompatibility, and biodegradability. Pepsin-soluble collagens were extracted from the skins of Oreochromis mossambicus (Om-PSC) and Gadus macrocephalus (Gm-PSC), and their structural properties and bioactivities were probed to reveal their potential applications in biomedical material for tissue engineering. The results of Fourier transforms-infrared spectroscopy (FT-IR), circular dichroism (CD), X-ray diffraction (XRD), ultraviolet (UV) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that Om-PSC and Gm-PSC had similar and intact triple helical structures. The amino acid composition and peptide profiles revealed Om-PSC and Gm-PSC were identified as type I collagen with the typical repetitive sequence of (Gly-X-Y) . However, the denaturation temperature (T) was determined to be 29.7 ℃ of Om-PSC, much higher than that of Gm-PSC (17.3 ℃). Toxicological experiments demonstrated Om-PSC and Gm-PSC both had good biocompatibility and cytocompatibility, which met the requirements of medical materials. Fluorescence imaging and cell cycle distribution revealed Om-PSC and Gm-PSC could promote the proliferation of fibroblast and osteoblast cells. Therefore, Om-PSC and Gm-PSC showed the advantages in medical materials.
水生胶原蛋白作为哺乳动物胶原蛋白的替代来源，由于其低成本、低抗原性、生物相容性和可生物降解性而受到越来越多的关注。从奥利亚罗非鱼（Om-PSC）和大头鳕（Gm-PSC）的皮中提取了胃蛋白酶可溶胶原蛋白，并研究了其结构特性和生物活性，以揭示其在组织工程生物医学材料中的潜在应用。傅里叶变换-红外光谱（FT-IR）、圆二色性（CD）、X 射线衍射（XRD）、紫外（UV）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）的结果表明，Om-PSC 和 Gm-PSC 具有相似且完整的三螺旋结构。氨基酸组成和肽图谱表明，Om-PSC 和 Gm-PSC 被鉴定为 I 型胶原蛋白，具有典型的（Gly-X-Y）重复序列。然而，Om-PSC 的变性温度（T）为 29.7℃，远高于 Gm-PSC（17.3℃）。毒理学实验表明，Om-PSC 和 Gm-PSC 均具有良好的生物相容性和细胞相容性，符合医用材料的要求。荧光成像和细胞周期分布表明，Om-PSC 和 Gm-PSC 均可促进成纤维细胞和成骨细胞的增殖。因此，Om-PSC 和 Gm-PSC 在医用材料方面具有优势。
