Chuy Gabriela Pereira, Muraro Pâmela Cristine Ladwig, Viana Altevir Rossato, Pavoski Giovani, Espinosa Denise Crocce Romano, Vizzotto Bruno Stefanello, da Silva William Leonardo
Biomedicine Course, Franciscan University, Santa Maria, RS Brazil.
Nanoscience Graduate Program, Franciscan University, Santa Maria, RS Brazil.
J Inorg Organomet Polym Mater. 2022;32(4):1213-1222. doi: 10.1007/s10904-021-02162-3. Epub 2021 Nov 23.
Antimicrobial resistance represents a serious concern to public health, being responsible for hospital infections, affecting mainly immunosuppressed patients. Thus, nanotechnology appears as an alternative to solve this problem, through the application of metallic nanoparticles with antimicrobial activity. The present work aims to synthesize and characterize AgNPs from (AgNPs-KP) and extract (AgNPs-AV), evaluating the antimicrobial activity against carbapenemase (KpC) and the cytotoxicity in the L929 cell line. AgNPs were prepared by the biosynthetic method using and were characterized by XRD, FTIR and SEM-EDS. Antimicrobial activity was tested using the MIC and MBC. The cytotoxicity was evaluated by the MTT method and neutral red. The production of ROS and nitrogen RNS tests were performed in the L929 cell line. Thus, it was possible to confirm the production of AgNPs-KP, through morphological, structural and elemental analysis. AgNPs from had potent antimicrobial activity in low concentration against antimicrobial resistant pathogens with MIC 9.76 µg mL and MBC 9.06 µg mL. Moreover, AgNPs-KP in concentrations of 10, 30 and 100 µg mL did not show cytotoxic properties for the L929 fibroblast, where only the cytotoxic effect was observed in high concentrations (300 µg mL). AgNPs-KP did not produce ROS about the analyzed concentrations and RNS production was only in the highest concentration of 3000 µg mL. Therefore, AgNPs biosynthesized by have potential medical applicability as a promising antimicrobial agent, using a simple and low-cost method, correlating nanomedicine as nanostructured materials.
抗菌耐药性是公共卫生领域的一个严重问题，它导致医院感染，主要影响免疫抑制患者。因此，纳米技术似乎是解决这一问题的一种选择，通过应用具有抗菌活性的金属纳米颗粒。本研究旨在从[具体物质]合成并表征银纳米颗粒（AgNPs-KP）和提取物（AgNPs-AV），评估其对碳青霉烯酶（KpC）的抗菌活性以及在L929细胞系中的细胞毒性。采用生物合成方法制备AgNPs，并通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜-能谱分析（SEM-EDS）对其进行表征。使用最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测试抗菌活性。通过MTT法和中性红评估细胞毒性。在L929细胞系中进行活性氧（ROS）和氮自由基（RNS）检测。因此，通过形态、结构和元素分析可以确认AgNPs-KP的生成。来自[具体物质]的AgNPs在低浓度下对耐药病原体具有强大的抗菌活性，MIC为9.76 μg/mL，MBC为9.06 μg/mL。此外，浓度为10、30和100 μg/mL的AgNPs-KP对L929成纤维细胞未显示细胞毒性，仅在高浓度（300 μg/mL）下观察到细胞毒性作用。在所分析的浓度下，AgNPs-KP未产生ROS，仅在最高浓度3000 μg/mL时产生RNS。因此，通过[具体物质]生物合成的AgNPs作为一种有前景的抗菌剂具有潜在的医学应用价值，采用了简单且低成本的方法，将纳米医学与纳米结构材料相关联。
