State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, 510275, Guangdong, China.
School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, Guangdong, China.
Antonie Van Leeuwenhoek. 2022 Jan;115(1):125-139. doi: 10.1007/s10482-021-01685-6. Epub 2021 Nov 29.
Bacterial symbionts of insects have been shown to play important roles in host fitness. However, little is known about the bacterial community of Tessaratoma papillosa which is one of the most destructive pests of the well-known fruits Litchi chinensis Sonn and Dimocarpus longan Lour in Oriental Region, especially in South-east Asia and adjacent areas. In this study, we surveyed the bacterial community diversity and dynamics of T. papillosa in all developmental stages with both culture-dependent and culture-independent methods by the third-generation sequencing technology. Five bacterial phyla were identified in seven developmental stages of T. papillosa. Proteobacteria was the dominant phylum and Pantoea was the dominant genus of T. papillosa. The results of alpha and beta diversity analyses showed that egg stage had the most complex bacterial community. Some of different developmental stages showed similarities, which were clustered into three phases: (1) egg stage, (2) early nymph stages (instars 1-3), and (3) late nymph stages (instars 4-5) and adult stage. Functional prediction indicated that the bacterial community played different roles in these three phases. Furthermore, 109 different bacterial strains were isolated and identified from various developmental stages. This study revealed the relationship between the symbiotic bacteria and the development of T. papillosa, and may thus contribute to the biological control techniques of T. papillosa in the future.
昆虫的共生细菌在宿主适应性方面起着重要作用。然而，对于荔枝蝽（Tessaratoma papillosa）的细菌群落，我们知之甚少。荔枝蝽是东方地区（尤其是东南亚和周边地区）著名水果荔枝（Litchi chinensis Sonn）和龙眼（Dimocarpus longan Lour）的最具破坏性害虫之一。在本研究中，我们采用培养依赖和培养非依赖的方法，通过第三代测序技术，调查了荔枝蝽在所有发育阶段的细菌群落多样性和动态。在荔枝蝽的七个发育阶段中，共鉴定出五个细菌门。变形菌门是优势门，泛菌属（Pantoea）是荔枝蝽的优势属。α多样性和β多样性分析的结果表明，卵期的细菌群落最复杂。不同发育阶段的一些结果相似，聚类为三个阶段：（1）卵期，（2）早期若虫期（1-3 龄）和（3）晚期若虫期（4-5 龄）和成虫期。功能预测表明，细菌群落在这三个阶段中发挥了不同的作用。此外，还从不同发育阶段分离和鉴定出 109 株不同的细菌株。本研究揭示了共生细菌与荔枝蝽发育的关系，可能有助于未来对荔枝蝽的生物防治技术。
