Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, 226026, India.
Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
Int J Nanomedicine. 2021 Nov 18;16:7711-7726. doi: 10.2147/IJN.S330763. eCollection 2021.
Protein-derived biogenic syntheses of inorganic nanoparticles have gained immense attention because of their broad spectrum of applications. Proteins offer a reducing environment to enable the synthesis of nanoparticles and encapsulate synthesized nanoparticles and provide them temporal stability in addition to biocompatibility.
In the present study, fruit proteins were used to synthesize silver nanoparticles (AgNPs) at 37 °C over five days of incubation. The synthesis of AgNPs was confirmed by UV-Vis spectroscopy, TEM, zeta potential, and DLS analyses. Further, these NPs depicted antibacterial and antibiofilm effects. Additionally, the anticancer activities of nanoparticles were also tested against the lung cancer cell line (A549) with respect to the normal cell line (NRK) using MTT assay. Further, the estimation of ROS generation through DCFH-DA staining along with a reduction in mitochondrial membrane potential by Mito Tracker Red CMX staining was carried out. Moreover, nuclear degradation in the AgNPs treated cells was cross-checked by DAPI staining.
The average size of AgNPs was detected to be 27 ±1 nm by TEM analysis, whereas surface encapsulation by protein was determined by FTIR spectroscopy. These NPs were effective against bacterial pathogens such as , and with MICs of 148.12 µg/mL, 165.63 µg/mL, 162.77 µg/mL, and 124.88 µg/mL, respectively. Furthermore, these nanoparticles inhibit the formation of biofilms of , and by 71.14%, 73.89%, 66.66%, and 64.81%, respectively. Similarly, these nanoparticles were also found to inhibit (IC50 = 57.11 µM) the lung cancer cell line (A549). At the same time, they were non-toxic against NRK cells up to a concentration of 200 µM.
We successfully synthesized potentially potent antibacterial, antibiofilm and anticancer biogenic AgNPs.
由于其广泛的应用，蛋白质衍生的生物合成无机纳米粒子引起了极大的关注。蛋白质提供了一个还原环境，使纳米粒子的合成成为可能，并对合成的纳米粒子进行封装，为其提供时间稳定性，同时保持生物相容性。
在本研究中，使用水果蛋白质在 37°C 下孵育五天来合成银纳米粒子（AgNPs）。通过紫外可见光谱、TEM、zeta 电位和 DLS 分析来确认 AgNPs 的合成。此外，这些纳米粒子表现出抗菌和抗生物膜的作用。此外，还使用 MTT 测定法针对肺癌细胞系（A549）测试了纳米粒子的抗癌活性，并与正常细胞系（NRK）进行了比较。此外，通过 DCFH-DA 染色评估 ROS 的产生，并用 Mito Tracker Red CMX 染色评估线粒体膜电位的降低。此外，通过 DAPI 染色检查 AgNPs 处理细胞中的核降解。
通过 TEM 分析检测到 AgNPs 的平均粒径为 27±1nm，而通过傅里叶变换红外光谱（FTIR）确定了蛋白质的表面包裹。这些纳米粒子对细菌病原体如 、 、 有效，MIC 值分别为 148.12μg/mL、165.63μg/mL、162.77μg/mL 和 124.88μg/mL。此外，这些纳米粒子还抑制了 、 生物膜的形成，抑制率分别为 71.14%、73.89%、66.66%和 64.81%。同样，这些纳米粒子也被发现抑制了肺癌细胞系（A549）的生长（IC50=57.11μM）。同时，它们在 200μM 浓度下对 NRK 细胞没有毒性。
我们成功地合成了具有潜在抗菌、抗生物膜和抗癌作用的生物合成 AgNPs。
