School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW, Australia.
SDx Tethered Membranes Pty Ltd, Sydney, NSW, Australia.
Methods Mol Biol. 2022;2402:61-69. doi: 10.1007/978-1-0716-1843-1_5.
Tethered bilayer lipid membranes (tBLMs) anchored to a solid substrate can be prepared and individual triangular voltage ramps from zero to 500 mV with a period of 2-10 ms applied to give membrane voltage dependencies with and without the addition of drugs and analytes in order to measure their electro-insertion properties.
将双层脂膜(tBLM)固定在固体基底上,可以施加从零到 500 mV 的三角形电压斜坡,周期为 2-10 ms,以测量药物和分析物的存在与否对膜电压的影响,从而测量它们的电插入特性。