酿酒酵母中的基因复制。
Gene duplication in Saccharomyces cerevisiae.
作者信息
Hansche P E, Beres V, Lange P
出版信息
Genetics. 1978 Apr;88(4 Pt 1):673-87.
Five independent duplications of the acid-phosphatase (aphtase) structural gene (acp1) were recovered from chemostat populations of S. cerevisiae that were subject to selection for in vivo hyper-aphtase activity. Two of the duplications arose spontaneously. Three of them were induced by UV. All five of the duplication events involved the transpositioning of the aphtase structural gene, acp1, and all known genes distal to acp1 on the right arm of chromosome II, to the terminus of an arm of other unknown chromosomes. One of the five duplicated regions of the right arm of chromosome II was found to be transmitted mitotically and meiotically with very high fidelity. The other four duplicated regions of the right arm of chromosome II were found to be unstable, being lost at a rate of about 2% per mitosis. However, selection for increased fidelity of mitotic transmission was effective in one of these strains. No tandem duplications of the aphtase structural gene were found.
从经体内高酸性磷酸酶活性筛选的酿酒酵母恒化器群体中，获得了酸性磷酸酶（aphtase）结构基因（acp1）的五个独立重复序列。其中两个重复序列是自发产生的。另外三个是由紫外线诱导产生的。所有五个重复事件都涉及酸性磷酸酶结构基因acp1以及位于第二条染色体右臂上acp1远端的所有已知基因，转位到其他未知染色体的一个臂的末端。发现第二条染色体右臂的五个重复区域之一在有丝分裂和减数分裂过程中以非常高的保真度传递。第二条染色体右臂的其他四个重复区域被发现是不稳定的，在每次有丝分裂时以约2%的速率丢失。然而，在其中一个菌株中，对提高有丝分裂传递保真度的选择是有效的。未发现酸性磷酸酶结构基因的串联重复。
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