Liu Yike, Zhu Guang, Zhu Zhangwang, Chen Lin, Niu Hongli, He Weijie, Tong Hanwen, Song Jinghan, Zhang Yuqing, Ma Dongfang, Gao Chunbao
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Engineering and Technology Research, Hubei Academy of Agricultural Sciences, Wuhan, China.
Center of Wheat, Wheat Disease Biology Research Station for Central China, Wuhan, China.
Front Plant Sci. 2021 Nov 17;12:777494. doi: 10.3389/fpls.2021.777494. eCollection 2021.
Fusarium seedling blight (FSB) is an important disease of wheat occurring as part of the Fusarium disease complex consisting also of Fusarium head blight (FHB). 240 Chinese elite cultivars and lines were evaluated in greenhouse experiments for FSB resistance and genotyped using the wheat 90 K single nucleotide polymorphism arrays. Among them, 23 accessions had an average lesion length of less than 0.6 cm, exhibiting potential for breeding for FSB resistance in wheat. Jingfumai 1 and Yangmai 11 had a relatively high resistance to both FSB and FHB simultaneously. Six relatively stable quantitative trait loci (QTLs) were detected on chromosome arms 1DL, 3AS, 3BL, 6BL, 7AL, and Un using the mixed linear model approach, interpreting 4.83-7.53% of phenotypic variation. There was a negative correlation between the average FSB lesion length and the BLUE FHB index with a low coefficient, and resistance to both diseases appeared to be conferred by different QTLs across the same population. Four KASP markers were detected on 1DL, 3AS, 3BL, and 6BL in QTLs to facilitate marker-assisted selection. Combined with transcriptome data analysis, eight defense-related genes were considered as candidates for mapping QTLs. The resistant elite germplasm, mapped QTLs, and KASP markers developed in this study are useful resources for enhancing Fusarium seedling blight in wheat breeding.
镰刀菌苗枯病（FSB）是小麦的一种重要病害，它是镰刀菌病害复合体的一部分，该病害复合体还包括镰刀菌穗腐病（FHB）。在温室试验中对240个中国优良品种和品系进行了FSB抗性评估，并使用小麦90K单核苷酸多态性阵列进行基因分型。其中，23份材料的平均病斑长度小于0.6厘米，表现出在小麦FSB抗性育种中的潜力。京福麦1号和扬麦11号对FSB和FHB同时具有较高抗性。使用混合线性模型方法在1DL、3AS、3BL、6BL、7AL染色体臂和Un上检测到6个相对稳定的数量性状位点（QTL），解释了4.83%-7.53%的表型变异。FSB平均病斑长度与BLUE FHB指数之间存在负相关，系数较低，并且同一群体中对两种病害的抗性似乎由不同的QTL赋予。在QTL的1DL、3AS、3BL和6BL上检测到4个KASP标记，以促进标记辅助选择。结合转录组数据分析，8个与防御相关的基因被认为是QTL定位的候选基因。本研究中开发的抗性优良种质、定位的QTL和KASP标记是增强小麦育种中镰刀菌苗枯病抗性的有用资源。
