Chaudhary Sidhant, Ricardo Rosa Margarida Nogueira, Dubey Mukesh, Jensen Dan Funck, Grenville-Briggs Laura, Karlsson Magnus
Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, SE-75007, Uppsala, Sweden.
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-23422, Lomma, Sweden.
G3 (Bethesda). 2024 Oct 7;14(12). doi: 10.1093/g3journal/jkae240.
Biological control to manage plant diseases is an environmentally friendly alternative to using chemical pesticides. However, little is known about the role of genetic variation in plants affecting the efficacy of biological control agents (BCAs). The aim of this study was to explore the genetic variation in winter wheat for disease susceptibility to fusarium foot rot caused by Fusarium graminearum and variation in biocontrol efficacy of the fungal BCA Clonostachys rosea to control the disease. In total, 190 winter wheat genotypes were evaluated under controlled conditions in two treatments, i.e. (i) F. graminearum (Fg) and (ii) F. graminearum infection on C. rosea treated seeds (FgCr). Alongside disease severity, plant growth-related traits such as shoot length and root length were also measured. Comparison of genotypes between the two treatments enabled the dissection of genotypic variation for disease resistance and C. rosea efficacy. The study revealed significant variation among plant genotypes for fusarium foot rot susceptibility and other growth traits in treatment Fg. Moreover, significant variation in C. rosea efficacy was also observed in genotype contrasts between the two treatments for all traits. Using a 20K marker array, a genome-wide association study was also performed. We identified a total of 18 significant marker-trait associations for disease resistance and C. rosea efficacy for all the traits. Moreover, the markers associated with disease resistance and C. rosea efficacy were not co-localized, highlighting the independent inheritance of these traits, which can facilitate simultaneous selection for cultivar improvement.
利用生物防治来管理植物病害是一种比使用化学农药更环保的选择。然而，关于植物遗传变异对生物防治剂（BCAs）功效的影响，我们所知甚少。本研究的目的是探究冬小麦对禾谷镰刀菌引起的赤霉病的感病性遗传变异，以及真菌生物防治剂粉红粘帚霉对该病的生物防治效果变异。总共190个冬小麦基因型在可控条件下进行了两种处理的评估，即（i）禾谷镰刀菌（Fg）处理和（ii）用粉红粘帚霉处理过的种子接种禾谷镰刀菌（FgCr）处理。除了病害严重程度外，还测量了与植物生长相关的性状，如地上部长度和根部长度。两种处理间基因型的比较有助于剖析抗病性和粉红粘帚霉功效的基因型变异。研究发现，在Fg处理中，不同植物基因型在赤霉病易感性和其他生长性状上存在显著变异。此外，在两种处理的基因型对比中，所有性状的粉红粘帚霉功效也存在显著变异。利用一个20K标记阵列，还进行了全基因组关联研究。我们总共鉴定出18个与抗病性和粉红粘帚霉功效相关的显著标记-性状关联。此外，与抗病性和粉红粘帚霉功效相关的标记并非共定位，这突出了这些性状的独立遗传，有助于同时选择以改良品种。
