Liu Juan, Gao Yang, Gong Feifei, Hou Feifan, Zhang Zhipeng, Cheng Xiaojing, Du Wei, Zhang Lingling, Wang Jinyao, Xu Jin, Xing Guoming, Kang Xiuping, Li Sen
College of Horticulture, Shanxi Agricultural University, Jinzhong, China.
Collaborative Innovation Center for Improving Quality and Increase of Protected Vegetables in Shanxi Province, Jinzhong, China.
Front Plant Sci. 2021 Nov 12;12:778956. doi: 10.3389/fpls.2021.778956. eCollection 2021.
Sulfur (S) fumigation is a commonly used sterilization method in horticultural facilities against fungal diseases. S fumigation damaged cucumber leaves, although the response mechanism is unclear. This study analyzes the growth, transcriptome, and metabolomic profiles of young and mature leaves, ovaries, and commercial cucumber fruits to decipher the mechanism of cucumber stress response under S fumigation. S fumigation significantly changed the photosynthetic efficiency and reactive oxygen species (ROS) in leaves, but not fruit development, fruit mass, and peel color. Transcriptome analysis indicated that S fumigation strongly regulated stress defense genes. The weighted gene co-expression network analysis revealed that S fumigation regulated , defense genes, , and protein kinase. The abscisic acid (ABA)-mediated model of regulation under S fumigation was constructed. Metabolome analysis showed that S fumigation significantly upregulated or downregulated the contents of amino acids, organic acids, sugars, glycosides, and lipids (VIP > 1 and -value < 0.05). The opposite Pearson's correlations of these differential metabolites implied that cucumber had different metabolic patterns in short-term and long-term S fumigation. Besides, the elevated levels of proline and triglyceride indicated that stress-responsive mechanisms existed in S-fumigated cucumber. Moreover, the comprehensive analysis indicated that S fumigation elevated secondary S-containing metabolites but decreased sulfate absorption and transportation in cucumber. Overall, our results provided a comprehensive assessment of S fumigation on cucumber, which laid the theoretical foundation for S fumigation in protected cultivation.
硫磺（S）熏蒸是园艺设施中常用的一种防治真菌病害的灭菌方法。硫磺熏蒸会损害黄瓜叶片，但其响应机制尚不清楚。本研究分析了幼叶和成熟叶、子房以及商品黄瓜果实的生长、转录组和代谢组谱，以阐明硫磺熏蒸下黄瓜应激反应的机制。硫磺熏蒸显著改变了叶片的光合效率和活性氧（ROS），但对果实发育、果实重量和果皮颜色没有影响。转录组分析表明，硫磺熏蒸强烈调控应激防御基因。加权基因共表达网络分析显示，硫磺熏蒸调控了 、防御基因、 和蛋白激酶。构建了硫磺熏蒸下脱落酸（ABA）介导的调控模型。代谢组分析表明，硫磺熏蒸显著上调或下调了氨基酸、有机酸、糖类、糖苷和脂质的含量（VIP>1且P值<0.05）。这些差异代谢物相反的皮尔逊相关性表明，黄瓜在短期和长期硫磺熏蒸下具有不同的代谢模式。此外，脯氨酸和甘油三酯水平的升高表明硫磺熏蒸的黄瓜中存在应激反应机制。此外，综合分析表明，硫磺熏蒸提高了黄瓜中含硫次生代谢物的含量，但降低了硫酸盐的吸收和运输。总体而言，我们的结果对硫磺熏蒸对黄瓜的影响进行了全面评估，为设施栽培中的硫磺熏蒸奠定了理论基础。
