Micusan V V, Mercier G, Bhatti A R, Reiser R F, Bergdoll M S, Oth D
Immunology. 1986 Jun;58(2):203-8.
Toxic shock syndrome toxin-1 (TSST-1), isolated from Staphylococcus aureus strains associated with toxic shock syndrome (TSS), is known as a potent mitogen and interleukin-1 inducer. The potential of TSST-1 as an interleukin-2 (IL-2) inducer was tested on human peripheral blood lymphocytes (HPBL) and murine spleen lymphocytes (MSL). These cells were incubated with TSST-1 and the supernatants analysed for IL-2 production. Preincubation of IL-2-dependent indicator cells (IC) with a monoclonal antibody specific for murine IL-2 receptors inhibited their proliferation by supernatants of TSST-1-treated MSL, thus strongly suggesting that they contain IL-2. The concentrations of TSST-1 required for HPBL or MSL to produce IL-2 ranged between 10(-1) and 10(-4) micrograms/ml. The amount of IL-2 units/ml varied little from one experiment to another. In contrast, IL-2 production by PHA-stimulated HPBL or Con A-stimulated MSL showed great variability and dependence on mitogen concentration. T-cell depleted MSL exposed to TSST-1 produced less IL-2. Experiments with germ-free mice and TSST-1-primed mice demonstrated that IL-2 production is not related to TSST-1 antigenicity.
从与中毒性休克综合征（TSS）相关的金黄色葡萄球菌菌株中分离出的中毒性休克综合征毒素-1（TSST-1），是一种强效的丝裂原和白细胞介素-1诱导剂。在人外周血淋巴细胞（HPBL）和小鼠脾淋巴细胞（MSL）上测试了TSST-1作为白细胞介素-2（IL-2）诱导剂的潜力。将这些细胞与TSST-1一起孵育，并分析上清液中IL-2的产生情况。用针对小鼠IL-2受体的单克隆抗体对IL-2依赖性指示细胞（IC）进行预孵育，可抑制其被TSST-1处理的MSL的上清液诱导的增殖，因此强烈表明上清液中含有IL-2。HPBL或MSL产生IL-2所需的TSST-1浓度在10^(-1)至10^(-4)微克/毫升之间。每次实验中每毫升IL-2单位的量变化不大。相比之下，PHA刺激的HPBL或Con A刺激的MSL产生的IL-2则表现出很大的变异性，且依赖于丝裂原浓度。暴露于TSST-1的T细胞耗竭的MSL产生的IL-2较少。对无菌小鼠和用TSST-1致敏的小鼠进行的实验表明，IL-2的产生与TSST-1的抗原性无关。
