Lafferty K J, Prowse S J, Al-Adra A, Warren H S, Vasalli J, Reich E
Aust J Exp Biol Med Sci. 1980 Dec;58(6):533-44. doi: 10.1038/icb.1980.55.
The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin-A blast cells given this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is interleukin 2. The presence of an inhibitor in the supernatant of mitogen activated lymphocytes and the species specificity of the factor are demonstrated.
活化T细胞的持续增殖需要培养基中存在淋巴细胞生长因子。本研究描述了一种快速、高度可重复的测定方法，用于定量测量这种淋巴因子的水平。使用伴刀豆球蛋白A母细胞使该测定具有高度的灵活性和便利性。结果表明，所测量的淋巴因子是白细胞介素2。证明了丝裂原活化淋巴细胞上清液中存在一种抑制剂以及该因子的种属特异性。
