脂肪组织来源的间充质干细胞通过其抗炎作用减少子宫内膜异位症细胞增殖。
Adipose tissue-derived mesenchymal stem cells reduce endometriosis cellular proliferation through their anti-inflammatory effects.
作者信息
Meligy Fatma Y, Elgamal Dalia A, Abdelzaher Lobna A, Khashbah Maha Y, El-Mokhtar Mohamed A, Sayed Ayat A, Refaiy Abeer M, Othman Essam R
机构信息
Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Reproductive Science Research Center, Faculty of Medicine, Assiut University, Assiut, Egypt.
出版信息
Clin Exp Reprod Med. 2021 Dec;48(4):322-336. doi: 10.5653/cerm.2021.04357. Epub 2021 Dec 1.
OBJECTIVE
Endometriosis is a chronic debilitating inflammatory condition characterized by the presence of endometrial tissues outside the uterine cavity. Pelvic soreness and infertility are the usual association. Due to the poor effectiveness of the hormone therapy and the high incidence of recurrence following surgical excision, there is no single effective option for management of endometriosis. Mesenchymal stem cells (MSCs) are multipotent stromal cells studied for their broad immunoregulatory and anti-inflammatory properties; however, their efficiency in endometriosis cases is still a controversial issue. Our study aim was to evaluate whether adipose tissue-derived MSCs (AD-MSCs) could help with endometriosis through their studied anti-inflammatory role.
METHODS
Female Wistar rats weighting 180 to 250 g were randomly divided into two groups: group 1, endometriosis group; established by transplanting autologous uterine tissue into rats' peritoneal cavities and group 2, stem cell treated group; treated with AD-MSCs on the 5th day after induction of endometriosis. The proliferative activity of the endometriosis lesions was evaluated through Ki67 staining. Quantitative estimation of interferon γ, tumor necrosis factor-α, interleukin (IL)-6, IL-1β, IL-10, and transforming growth factor β expression, as well as immunohistochemical detection of CD68 positive macrophages, were used to assess the inflammatory status.
RESULTS
The size and proliferative activity of endometriosis lesions were significantly reduced in the stem cell treated group. Stem cells efficiently mitigated endometriosis associated chronic inflammatory reactions estimated through reduction of CD68 positive macrophages and the expression of the proinflammatory cytokines.
CONCLUSION
Stem cell therapy can be considered a novel remedy in endometriosis possibly through its anti-inflammatory and antiproliferative properties.
目的
子宫内膜异位症是一种慢性衰弱性炎症性疾病，其特征是子宫腔外存在子宫内膜组织。盆腔疼痛和不孕是常见的关联症状。由于激素治疗效果不佳以及手术切除后复发率高，目前尚无单一有效的子宫内膜异位症治疗方案。间充质干细胞（MSCs）是具有多能性的基质细胞，因其广泛的免疫调节和抗炎特性而受到研究；然而，它们在子宫内膜异位症病例中的疗效仍是一个有争议的问题。我们的研究目的是评估脂肪组织来源的间充质干细胞（AD-MSCs）是否能通过其已被研究的抗炎作用来帮助治疗子宫内膜异位症。
方法
将体重180至250克的雌性Wistar大鼠随机分为两组：第1组为子宫内膜异位症组，通过将自体子宫组织移植到大鼠腹腔内建立；第2组为干细胞治疗组，在子宫内膜异位症诱导后第5天用AD-MSCs进行治疗。通过Ki67染色评估子宫内膜异位症病变的增殖活性。采用定量估计干扰素γ、肿瘤坏死因子-α、白细胞介素（IL）-6、IL-1β、IL-10和转化生长因子β的表达，以及免疫组织化学检测CD68阳性巨噬细胞，来评估炎症状态。
结果
干细胞治疗组中子宫内膜异位症病变的大小和增殖活性显著降低。通过减少CD68阳性巨噬细胞和促炎细胞因子的表达估计，干细胞有效减轻了与子宫内膜异位症相关的慢性炎症反应。
结论
干细胞疗法可能因其抗炎和抗增殖特性，被认为是治疗子宫内膜异位症的一种新方法。
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