Institute for Health Research, Epidemiological Surveillance and Training (IRESSEF), Dakar, Senegal.
Abbott Global Surveillance Program, Abbott Laboratories, Abbott Park, IL, USA.
Sci Rep. 2021 Dec 8;11(1):23644. doi: 10.1038/s41598-021-02874-z.
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineages that carry mutations in the spike gene are of concern for potential impact to treatment and prevention efforts. To monitor for new SARS-CoV-2 mutations, a panel of specimens were sequenced from both wave one (N = 96), and wave two (N = 117) of the pandemic in Senegal by whole genome next generation sequencing. Amongst these genomes, new combinations of SARS-CoV-2 spike mutations were identified, with E484K + N501T, L452R + N501Y, and L452M + S477N exclusively found in second wave specimens. These sequences are evidence of local diversification over the course of the pandemic and parallel evolution of escape mutations in different lineages.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）谱系的出现，其刺突基因发生突变，这令人担忧可能会对治疗和预防工作产生影响。为了监测新的 SARS-CoV-2 突变，通过全基因组下一代测序对塞内加尔大流行的第一波（N=96）和第二波（N=117）的一组样本进行了测序。在这些基因组中，发现了 SARS-CoV-2 刺突突变的新组合，第二波样本中仅发现 E484K+N501T、L452R+N501Y 和 L452M+S477N。这些序列证明了在大流行过程中本地多样化，以及不同谱系中逃避突变的平行进化。
