Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Kolkata, West Bengal, 741249, India.
Department of Applied Biology, School of Biological Sciences, University of Science and Technology Meghalaya, Ri-Bhoi, 793101, India.
J Mater Sci Mater Med. 2021 Dec 11;32(12):151. doi: 10.1007/s10856-021-06612-9.
This study employed a bottom-up technique to synthesize copper oxide (CuO) nanoparticles over hydrophilic graphene oxide (GO) nanosheets. The CuO/GO nanocomposite has been prepared using two selected precursors of copper nitrate and citric acid with an intermittent mixing of GO solutions. The synthesized Nanocomposites were characterized using different biophysical techniques like FT-IR, NMR, FE-SEM, and HR-TEM analyses. FT-IR analyses confirm the nanocomposites' successful formation, which is evident from the functional groups of C=C, C-O, and Cu-C stretching vibrations. Morphological analyses reveal the depositions of CuO nanoparticles over the planar rough GO sheets, which has been elucidated from the FE-SEM and HR-TEM analyses supported by respective EDAX analyses. The antimicrobial activities have been evident from the surface roughness and damages seen from the FE-SEM analyses. The CuO/GO sheets were tested against Gram-positive (e.g., Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa). It is evident that the intrinsic antibacterial activity of CuO/GO sheets, when combined in equal proportions, elicited a robust antibacterial activity when tested over Gram -ve representative bacteria Escherichia coli. The antioxidant behaviour of synthesized CuO/GO nanocomposite was evaluated by scavenging the free radicals of DPPH and ABTS. Moreover, the cytotoxic activity was also studied against epidermoid carcinoma cell line A-431. A brief mathematical formulation has been proposed in this study to uncover the possibilities of using the nanocomposites as potential drug candidates in theranostic applications in disease treatment and diagnosis. This study would help uncover the electronic properties that play in the nano-scaled system at the material-bio interface, which would aid in designing a sensitive nano-electromechanical device bearing both the therapeutic and diagnostic attributes heralding a new horizon in the health care systems.
本研究采用自下而上的技术，在亲水性氧化石墨烯（GO）纳米片上合成氧化铜（CuO）纳米粒子。使用硝酸铜和柠檬酸两种选定的前体，通过间歇混合 GO 溶液制备了 CuO/GO 纳米复合材料。使用不同的生物物理技术，如 FT-IR、NMR、FE-SEM 和 HR-TEM 分析对合成的纳米复合材料进行了表征。FT-IR 分析证实了纳米复合材料的成功形成，这可以从 C=C、C-O 和 Cu-C 伸缩振动的功能基团中明显看出。形态分析表明，CuO 纳米粒子沉积在平面粗糙的 GO 片上，这从 FE-SEM 和 HR-TEM 分析中得到了阐明，并得到了各自的 EDAX 分析的支持。从 FE-SEM 分析中看到的表面粗糙度和损伤可以看出抗菌活性。CuO/GO 片被测试了对抗革兰氏阳性（如金黄色葡萄球菌）和革兰氏阴性（大肠杆菌、铜绿假单胞菌）细菌。当以相等的比例组合时，CuO/GO 片的固有抗菌活性显然会在测试革兰氏阴性代表细菌大肠杆菌时引起强烈的抗菌活性。通过清除 DPPH 和 ABTS 的自由基评估了合成的 CuO/GO 纳米复合材料的抗氧化行为。此外，还研究了对表皮癌细胞系 A-431 的细胞毒性。本研究提出了一个简单的数学公式，以揭示将纳米复合材料用作治疗和诊断疾病的治疗应用中的潜在药物候选物的可能性。这项研究将有助于揭示在材料-生物界面处的纳米尺度系统中起作用的电子特性，这将有助于设计具有治疗和诊断属性的灵敏纳米机电设备，为医疗保健系统开辟新的视野。
