Bio-nanomaterials Research Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi, 630003, Tamil Nadu, India.
Bio-nanomaterials Research Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi, 630003, Tamil Nadu, India.
Int J Biol Macromol. 2019 Oct 15;139:867-878. doi: 10.1016/j.ijbiomac.2019.07.214. Epub 2019 Jul 31.
In this study biopolymer-inorganic material of chitosan‑copper oxide-neem seed (CS-CuO-NS) biocomposite was successfully synthesized by simple precipitation method and characterized by FT-IR, XRD, HR-SEM, TEM and TGA analyses. From HR-SEM and TEM analysis, CS-CuO-NS biocomposite shows flower and needle like structure respectively. The size of the as prepared CS-CuO-NS biocomposite is found to be 20-100 nm. All the synthesized materials were tested for antibacterial activity against both gram positive like Staphylococcus aureus (S. aureus) and Streptococcus pyogenes (S. pyogenes) and gram negative like Escherichia coli (E. coli) and Klebsiella aerogenes (K. aerogenes) bacterial strains. The maximum zone of inhibition is obtained for CS-CuO-NS biocomposite against S. aureus (23 mm), S. pyogenes (21 mm), E. coli (22 mm) and K. aerogenes (20 mm). The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. The antioxidant activity was determined by free radicals scavenging such as 1, 1-Diphenyl-2-picryhydrazyl (DPPH) and 2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Furthermore, the cytotoxicity effect was investigated against human breast cancer (MCF-7) cell line and the highest cytotoxicity (IC:16.33 μg/mL) is found to be in biocomposite. From the results of antibacterial, antioxidant and cytotoxic activities, it is concluded that CS-CuO-NS biocomposite may be suitable for biomedical applications.
在这项研究中，通过简单的沉淀法成功合成了壳聚糖-氧化铜-印楝种子（CS-CuO-NS）生物复合材料，并通过 FT-IR、XRD、HR-SEM、TEM 和 TGA 分析进行了表征。从 HR-SEM 和 TEM 分析可以看出，CS-CuO-NS 生物复合材料分别呈现出花状和针状结构。所制备的 CS-CuO-NS 生物复合材料的尺寸为 20-100nm。所有合成材料均测试了对革兰氏阳性菌（金黄色葡萄球菌（S. aureus）和化脓性链球菌（S. pyogenes））和革兰氏阴性菌（大肠杆菌（E. coli）和产气克雷伯菌（K. aerogenes））的抗菌活性。CS-CuO-NS 生物复合材料对 S. aureus（23mm）、S. pyogenes（21mm）、E. coli（22mm）和 K. aerogenes（20mm）的抑菌圈最大。测定了最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。通过自由基清除（如 1,1-二苯基-2-苦基肼（DPPH）和 2,2-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS））测定了抗氧化活性。此外，还研究了对人乳腺癌（MCF-7）细胞系的细胞毒性作用，发现生物复合材料的最高细胞毒性（IC：16.33μg/mL）。从抗菌、抗氧化和细胞毒性活性的结果来看，CS-CuO-NS 生物复合材料可能适用于生物医学应用。
