Instituto de Virología e Innovaciones Tecnológicas (IVIT, INTA-CONICET), Hurlingham, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; Instituto de Innovación para La Producción Agropecuaria y El Desarrollo Sostenible (IPADS Balcarce), INTA-CONICET, Balcarce, Buenos Aires, Argentina.
Virology. 2022 Jan;566:98-105. doi: 10.1016/j.virol.2021.11.014. Epub 2021 Dec 3.
The innate and acquired immune response induced by a commercial inactivated vaccine against Bovine Herpesvirus-1 (BoHV-1) and protection conferred against the virus were analyzed in cattle. Vaccination induced high levels of BoHV-1 antibodies at 30, 60, and 90 days post-vaccination (dpv). IgG1 and IgG2 isotypes were detected at 90 dpv, as well as virus-neutralizing antibodies. An increase of anti-BoHV-1 IgG1 in nasal swabs was detected 6 days post-challenge in vaccinated animals. After viral challenge, lower virus excretion and lower clinical score were observed in vaccinated as compared to unvaccinated animals, as well as BoHV-1-specific proliferation of lymphocytes and production of IFNγ, TNFα, and IL-4. Downregulation of the expression of endosome Toll-like receptors 8-9 was detected after booster vaccination. This is the first thorough study of the immunity generated by a commercial vaccine against BoHV-1 in cattle.
本研究分析了商业化牛疱疹病毒 1 型（BoHV-1）灭活疫苗诱导的牛固有和获得性免疫应答及其对病毒的保护作用。疫苗接种后 30、60 和 90 天可诱导高水平的 BoHV-1 抗体。在 90 天 dpv 时检测到 IgG1 和 IgG2 同种型以及病毒中和抗体。在接种动物中，攻毒后 6 天可检测到鼻拭子中抗 BoHV-1 IgG1 的增加。与未接种疫苗的动物相比，接种疫苗的动物在病毒攻毒后病毒排出量和临床评分均较低，同时淋巴细胞的 BoHV-1 特异性增殖和 IFNγ、TNFα 和 IL-4 的产生也增加。在加强免疫后，检测到内体 Toll 样受体 8-9 的表达下调。这是首次对商业化 BoHV-1 疫苗在牛中产生的免疫进行全面研究。
