Servicio de Microbiología, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain.
Methods Mol Biol. 2022;2420:207-216. doi: 10.1007/978-1-0716-1936-0_16.
MALDI-TOF mass spectrometry has been applied with huge success to the identification of cultured microorganisms in clinical microbiology laboratories where it has become the reference method because it is simple, fast, and highly reproducible. We describe the different procedures used in the routine for pathogen identification using the Bruker MALDI Biotyper system.
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)已被广泛应用于临床微生物学实验室中培养微生物的鉴定,它已成为参考方法,因为它简单、快速且具有高度可重复性。我们描述了使用 Bruker MALDI Biotyper 系统进行病原体鉴定的常规程序中使用的不同步骤。