Gromkova R, Goodgal S
J Bacteriol. 1981 Apr;146(1):79-84. doi: 10.1128/jb.146.1.79-84.1981.
The uptake of circular and linear plasmid RSF0885 deoxyribonucleic acids, (DNAs) obtained from Haemophilus parainfluenzae 14, in both homologous and heterologous recipients was studied and compared with that of chromosomal DNA. High concentrations of divalent cations stimulated the uptake of either circular or linear plasmid DNA in H. parainfluenzae 14 competent cells but did not affect the uptake of chromosomal DNA. The biological activity of linear plasmid DNA was similar to that of circular DNA, and the transforming efficiencies for ampicillin resistance of both molecular forms were stimulated by divalent ions. Plasmid DNA was taken up efficiently either with or without the addition of divalent ions but was not biologically active in the heterologous Haemophilus influenzae Rd recipient. Our results suggest that in H. parainfluenzae 14 some of the steps for chromosomal and plasmid DNA uptake are different.
对从副流感嗜血杆菌14中获得的环状和线性质粒RSF0885脱氧核糖核酸(DNA)在同源和异源受体中的摄取情况进行了研究,并与染色体DNA的摄取情况进行了比较。高浓度的二价阳离子刺激了副流感嗜血杆菌14感受态细胞中环状或线性质粒DNA的摄取,但不影响染色体DNA的摄取。线性质粒DNA的生物活性与环状DNA相似,两种分子形式对氨苄青霉素抗性的转化效率均受到二价离子的刺激。无论是否添加二价离子,质粒DNA都能被有效摄取,但在异源流感嗜血杆菌Rd受体中无生物活性。我们的结果表明,在副流感嗜血杆菌14中,染色体DNA和质粒DNA摄取的一些步骤是不同的。