Zhou Mi, Ghoshal Bibaswan, Stothard Paul, Guan Le Luo
Department of Agricultural, Food and Nutritional Science, 416F Agriculture/Forestry center, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Centre for Addiction and Mental Health, 33 Ursula Franklin St, Toronto, ON M5S 2S1, Canada.
Curr Res Microb Sci. 2021 Nov 28;2:100085. doi: 10.1016/j.crmicr.2021.100085. eCollection 2021 Dec.
Rumen content-associated (RC) and epithelial tissue-attached (RT) bacterial communities are composed of different phylotypes and play distinctive roles. This study aimed to compare the composition of the RT and RC bacterial communities of steers differing in feed efficiency. The microbiota of RT and RC samples collected from sixteen beef steers with high or low residual feed intake (RFI) were analyzed through sequencing of partial 16S rRNA gene amplicons. and were the predominant phyla and was the most abundant genus in both RC and RT bacterial communities. In total, 19 OTUs of the RC samples and 19 OTUs of the RT samples were differentially abundant (DA) between H-RFI and l-RFI steers. Among them, a common DA OTU belonged to genus was identified in both RC and RT samples, making it the potential key microbial marker for indicating feed efficiency of steers. The co-occurrence of the DA OTUs among RT and RC samples suggest the importance of these two communities function as a complete system in influencing host feed efficiency.
瘤胃内容物相关（RC）和上皮组织附着（RT）细菌群落由不同的系统发育型组成，并发挥着独特的作用。本研究旨在比较饲料效率不同的阉牛的RT和RC细菌群落组成。通过对部分16S rRNA基因扩增子进行测序，分析了从16头高或低残余采食量（RFI）的肉牛阉牛中采集的RT和RC样本的微生物群。在RC和RT细菌群落中，厚壁菌门和拟杆菌门是主要门类，瘤胃球菌属是最丰富的属。总体而言，在高RFI和低RFI阉牛之间，RC样本的19个操作分类单元（OTU）和RT样本的19个OTU丰度存在差异（DA）。其中，在RC和RT样本中均鉴定出一个属于瘤胃球菌属的常见DA OTU，使其成为指示阉牛饲料效率的潜在关键微生物标志物。RT和RC样本中DA OTU的共现表明，这两个群落作为一个完整系统在影响宿主饲料效率方面具有重要性。
