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一种用于快速灵敏检测免疫抑制剂的集成装置。

An integrated device for fast and sensitive immunosuppressant detection.

机构信息

Institute of Applied Physics "Nello Carrara", CNR-IFAC, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.

Institute for Electromagnetic Sensing of the Environment, CNR-IREA, Via Diocleziano 328, 80124, Napoli, Italy.

出版信息

Anal Bioanal Chem. 2022 Apr;414(10):3243-3255. doi: 10.1007/s00216-021-03847-x. Epub 2021 Dec 22.

DOI:10.1007/s00216-021-03847-x
PMID:34936009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8956524/
Abstract

The present paper describes a compact point of care (POC) optical device for therapeutic drug monitoring (TDM). The core of the device is a disposable plastic chip where an immunoassay for the determination of immunosuppressants takes place. The chip is designed in order to have ten parallel microchannels allowing the simultaneous detection of more than one analyte with replicate measurements. The device is equipped with a microfluidic system, which provides sample mixing with the necessary chemicals and pumping samples, reagents and buffers into the measurement chip, and with integrated thin film amorphous silicon photodiodes for the fluorescence detection. Submicrometric fluorescent magnetic particles are used as support in the immunoassay in order to improve the efficiency of the assay. In particular, the magnetic feature is used to concentrate the antibody onto the sensing layer leading to a much faster implementation of the assay, while the fluorescent feature is used to increase the optical signal leading to a larger optical dynamic change and consequently a better sensitivity and a lower limit of detection. The design and development of the whole integrated optical device are here illustrated. In addition, detection of mycophenolic acid and cyclosporine A in spiked solutions and in microdialysate samples from patient blood with the implemented device are reported.

摘要

本文介绍了一种用于治疗药物监测（TDM）的紧凑型即时检测（POC）光学设备。该设备的核心是一种一次性塑料芯片，其中进行用于测定免疫抑制剂的免疫测定。该芯片的设计目的是具有十个平行的微通道，允许同时检测多个分析物并进行重复测量。该设备配备了微流控系统，可提供与必要化学品的样品混合，并将样品、试剂和缓冲液泵入测量芯片，以及集成的薄膜非晶硅光电二极管用于荧光检测。亚微米荧光磁性颗粒在免疫测定中用作支持物，以提高测定效率。特别是，磁性特征用于将抗体浓缩到传感层上，从而实现更快的测定实施，而荧光特征用于增加光学信号，从而导致更大的光学动态变化，从而提高灵敏度和更低的检测限。这里说明了整个集成光学设备的设计和开发。此外，还报告了使用所实现的设备在加标溶液中和从患者血液中的微透析样品中检测麦考酚酸和环孢素 A。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/dd4678c86260/216_2021_3847_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/5819f2721860/216_2021_3847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/f11fbed43618/216_2021_3847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/b7234189ffa6/216_2021_3847_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/bf69b3b696b2/216_2021_3847_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/abe45a5ca0ca/216_2021_3847_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/4739b5bec71e/216_2021_3847_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/25ed0992e863/216_2021_3847_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/dd4678c86260/216_2021_3847_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/5819f2721860/216_2021_3847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/f11fbed43618/216_2021_3847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/b7234189ffa6/216_2021_3847_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/bf69b3b696b2/216_2021_3847_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/abe45a5ca0ca/216_2021_3847_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/4739b5bec71e/216_2021_3847_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/25ed0992e863/216_2021_3847_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71aa/8956524/dd4678c86260/216_2021_3847_Fig8_HTML.jpg

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