State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University , Hangzhou, China.
Zhejiang Provincial Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University , Hangzhou, China.
Virulence. 2020 Dec;11(1):1685-1700. doi: 10.1080/21505594.2020.1848983.
The process of protein translocation into the endoplasmic reticulum (ER) is the initial and decisive step in the biosynthesis of all secretory proteins and many soluble organelle proteins. In this process, the Sec61 complex is the protein-conducting channel for transport. In this study, we identified and characterized the β subunit of the Sec61 complex in (MoSec61β). Compared with the wild-type strain Guy11, the Δ mutant exhibited highly branched mycelial morphology, reduced conidiation, high sensitivity to cell wall integrity stress, severely reduced virulence to rice and barley, and restricted biotrophic invasion. The turgor pressure of Δ was notably reduced, which affected the function of appressoria. Moreover, Δ was also sensitive to oxidative stress and exhibited a reduced ability to overcome plant immunity. Further examination demonstrated that MoSec61β affected the normal secretion of the apoplastic effectors Bas4 and Slp1. In addition, Δ upregulated the level of ER-phagy. In conclusion, our results demonstrate the importance of the roles played by MoSec61β in the fungal development and pathogenesis of .
蛋白质易位进入内质网（ER）的过程是所有分泌蛋白和许多可溶性细胞器蛋白生物合成的初始和决定性步骤。在此过程中，Sec61 复合物是运输的蛋白导通道。在这项研究中，我们鉴定并表征了 （MoSec61β）中的 Sec61 复合物的 β 亚基。与野生型菌株 Guy11 相比，Δ 突变体表现出高度分支的菌丝形态、减少的分生孢子形成、对细胞壁完整性应激的高敏感性、对水稻和大麦的毒力严重降低以及限制了生物营养入侵。Δ 的膨压明显降低，这影响了附着胞的功能。此外，Δ 还对氧化应激敏感，并表现出降低的克服植物免疫的能力。进一步的检查表明，MoSec61β 影响了质外体效应物 Bas4 和 Slp1 的正常分泌。此外，Δ 上调了 ER 自噬的水平。总之，我们的结果表明 MoSec61β 在 的真菌发育和发病机制中起着重要作用。
