Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Front Immunol. 2021 Dec 7;12:739591. doi: 10.3389/fimmu.2021.739591. eCollection 2021.
Vaccines are safe and efficient in controlling bacterial diseases in the aquaculture industry and are in line with green farming. The present study develops a previously unreported approach to prepare a live-attenuated vaccine by culturing bacteria in a high concentration of magnesium to attenuate bacterial virulence. Furthermore, metabolomes of zebrafish immunized with the live-attenuated vaccines were compared with those of survival and dying zebrafish infected by . The enhanced TCA cycle and increased fumarate were identified as the most key metabolic pathways and the crucial biomarker of vaccine-mediated and survival fish, respectively. Exogenous fumarate promoted expression of , , , , and lysozyme in a dose-dependent manner. Among the five innate immune genes, the elevated , , and are overlapped in the vaccine-immunized zebrafish and the survival from the infection. These findings highlight a way in development of vaccines and exploration of the underlying mechanisms.
疫苗在水产养殖中控制细菌性疾病是安全有效的，符合绿色养殖。本研究开发了一种以前未报道的方法，通过在高浓度镁中培养细菌来减弱细菌的毒力，从而制备减毒活疫苗。此外，用减毒活疫苗免疫斑马鱼的代谢组学与 感染的存活和垂死斑马鱼的代谢组学进行了比较。增强的 TCA 循环和增加的富马酸被确定为疫苗介导和存活鱼类的最关键代谢途径和关键生物标志物。外源性富马酸以剂量依赖的方式促进 、 、 、 和溶菌酶的表达。在这五个先天免疫基因中，疫苗免疫的斑马鱼和从感染中存活的斑马鱼中上调的 、 、 和 是重叠的。这些发现强调了一种疫苗开发和潜在机制探索的方法。
