Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Sci Rep. 2021 Dec 24;11(1):24419. doi: 10.1038/s41598-021-03031-2.
In this research, a new nano drug-based multi-walled carbon nanotubes (MWCNTs) was prepared and evaluated qualitatively. Bromocriptine (BRC) was conjugated to functionalized carbon nanotubes. Then, the CHNS, FT-IR, SEM, and RAMAN tests for characterization of the conjugated drug were done. The nanofluid-containing nano-drug was evaluated on lung cancer cells (A549 & QU-DB) and MRC5 by MTT and flow cytometry tests. Then, the gene expression studies of dopamine receptor genes were done before and after nano-drug treatment. After that, a western blotting test was carried out for further investigation of dopamine receptors protein production. Finally, Bax and Bcl-2 secretion were measured by the ELISA method in cells affected by MWCNTs-BRC Nf compared to untreated cells. The results showed that the nano-drug had a significant lethal effect on cancer cells, while it had no toxicity on MRC5. Also, the nano-drug could significantly induce apoptosis in lung cancer cells at a lower dose compared to the drug alone. In this study, a targeted nano-drug delivery system was designed, and its performance was evaluated based on neurotransmitter pathways, and the results showed that it may be useful in the treatment of lung cancer. However, additional studies on animal models are underway.
在这项研究中，制备并定性评估了一种基于新型纳米药物的多壁碳纳米管 (MWCNTs)。将溴隐亭 (BRC) 与功能化碳纳米管连接。然后，对结合药物进行 CHNS、FT-IR、SEM 和 RAMAN 测试进行表征。通过 MTT 和流式细胞术试验评估含纳米流体的纳米药物对肺癌细胞 (A549 和 QU-DB) 和 MRC5 的影响。然后，在纳米药物治疗前后进行多巴胺受体基因的表达研究。之后，通过 Western blot 试验进一步研究多巴胺受体蛋白的产生。最后，通过 ELISA 法测量受 MWCNTs-BRC Nf 影响的细胞与未处理细胞中 Bax 和 Bcl-2 的分泌。结果表明，纳米药物对癌细胞具有显著的致死作用，而对 MRC5 则没有毒性。此外，与单独使用药物相比，纳米药物在较低剂量下可显著诱导肺癌细胞凋亡。在这项研究中，设计了一种靶向纳米药物传递系统，并基于神经递质途径评估了其性能，结果表明它可能对肺癌的治疗有用。然而，正在进行动物模型的进一步研究。
