Kleine B, Sprenger R, Martinez-Alonso C, Bessler W G
Immunology. 1987 May;61(1):29-34.
The reactivity of 38 murine strains to a synthetic analogue of bacterial lipoprotein, tripalmitoyl-pentapeptide (TPP), was tested and compared with the reactivity to lipopolysaccharide (LPS). These strains include common laboratory mice and H-2 recombinant inbred lines, as well as some newly bred lines originating from animals recently captured in different regions of Europe. All animals analysed were reactive to TPP and polyclonally activated to proliferation and immunoglobulin synthesis. Large differences in mitogen reactivities of various H-2 recombinant inbred strains suggest that MHC or closely linked gene products influence the reactivity to the LPS and TPP mitogens. By analysing the frequencies of precursor cells reactive to TPP or LPS and the isotype patterns obtained after stimulation, we demonstrated that both mitogens activate individual B cells in different ways.
测试了38种小鼠品系对细菌脂蛋白合成类似物三棕榈酰五肽（TPP）的反应性，并与对脂多糖（LPS）的反应性进行了比较。这些品系包括常见的实验室小鼠和H-2重组近交系，以及一些源自最近在欧洲不同地区捕获的动物的新培育品系。所有分析的动物对TPP均有反应，并被多克隆激活以进行增殖和免疫球蛋白合成。各种H-2重组近交系在丝裂原反应性上存在很大差异，这表明主要组织相容性复合体（MHC）或紧密连锁的基因产物会影响对LPS和TPP丝裂原的反应性。通过分析对TPP或LPS有反应的前体细胞频率以及刺激后获得的同种型模式，我们证明这两种丝裂原以不同方式激活单个B细胞。
