Attik Nina, Colon Pierre, Gauthier Rémy, Chevalier Charlène, Grosgogeat Brigitte, Abouelleil Hazem
Université de Lyon - Université Claude Bernard Lyon 1, UMR CNRS 5615, Laboratoire des Multimatériaux et Interfaces, Cedex 08, Lyon, 69372 France; Université de Lyon, Université Claude Bernard Lyon 1, Faculté d'Odontologie, Cedex 08, Lyon, 69372 France.
Université de Lyon - Université Claude Bernard Lyon 1, UMR CNRS 5615, Laboratoire des Multimatériaux et Interfaces, Cedex 08, Lyon, 69372 France; Assistance Publique-Hôpitaux de Paris, Hôpital Rothschild, Service d'Odontologie, Université de Paris, Faculté dentaire, Paris, France.
Dent Mater. 2022 Feb;38(2):e19-e30. doi: 10.1016/j.dental.2021.12.029. Epub 2021 Dec 24.
To evaluate in vitro the mechanical, biological, and polymerization behavior of a flowable bulk-fill composite with fibers as a dispersed phase.
EverX Flow™ (GC Corporation) (EXF), one conventional bulk-fill composite (Filtek™ Bulk Fill Posterior Restorative, 3 M (FBF)), and one flowable bulk composite without fibers (SDR® flow+, Dentsply (SDR)) were tested. Samples were characterized in terms of flexural strength (ISO 4049), fracture toughness (ISO 20795-1), and Vickers hardness. Polymerization stress and volumetric shrinkage were evaluated. The in vitro biological assessment was achieved on cultured primary Human Gingival Fibroblast cells (HGF). The cell metabolic activity was evaluated using Alamar Blue assay at 1, 3, and 5 days of contact to the 3 tested composite extracts (ISO 10993) and cell morphology was evaluated by confocal microscopy. Data were submitted to One-Way analysis of variance (ANOVA) and independent t-test (α = 0.05).
FBF showed statistically higher Vickers hardness and flexural modulus than EXF and SDR. However, EXF showed statistically higher K than FBF and SDR. EXF had the statistically highest shrinkage stress values and FBF the lowest. Archimedes volumetric shrinkage showed significantly lower values for FBF as compared to the other two composites. Slight cytotoxic effect was observed for the three composites at day one. An enhancement of metabolic activity at day 5 was observed in cells treated with EXF extracts.
EXF had a significantly higher fracture toughness validating its potential use as a restorative material in stress bearing areas. EXF showed higher shrinkage stress values, and less cytotoxic effect. Fiber reinforced flowable composite is mainly indicated for deep and large cavities, signifying the importance for assessing its shrinkage stress and biological behavior.
在体外评估一种以纤维为分散相的可流动大块充填复合树脂的机械性能、生物学性能及聚合行为。
测试了EverX Flow™（GC公司）（EXF）、一种传统大块充填复合树脂（Filtek™ Bulk Fill Posterior Restorative，3M公司（FBF））和一种无纤维的可流动大块复合树脂（SDR® flow+，登士柏公司（SDR））。对样品进行了弯曲强度（ISO 4049）、断裂韧性（ISO 20795-1）和维氏硬度的表征。评估了聚合应力和体积收缩率。在培养的原代人牙龈成纤维细胞（HGF）上进行了体外生物学评估。在与3种测试复合树脂提取物接触1、3和5天时，使用阿拉玛蓝法评估细胞代谢活性，并通过共聚焦显微镜评估细胞形态。数据进行单因素方差分析（ANOVA）和独立t检验（α = 0.05）。
FBF的维氏硬度和弯曲模量在统计学上高于EXF和SDR。然而，EXF的断裂韧性在统计学上高于FBF和SDR。EXF的收缩应力值在统计学上最高，FBF最低。阿基米德体积收缩率显示，与其他两种复合树脂相比，FBF的值显著更低。在第1天时，观察到三种复合树脂均有轻微的细胞毒性作用。在用EXF提取物处理的细胞中，在第5天时观察到代谢活性增强。
EXF具有显著更高的断裂韧性，证实了其在应力承载区域作为修复材料的潜在用途。EXF显示出更高的收缩应力值和更低的细胞毒性作用。纤维增强可流动复合树脂主要适用于深大窝洞，这表明评估其收缩应力和生物学行为的重要性。
