Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China.
Division of Agriculture, Department of Animal Science, University of Arkansas, Fayetteville, Arkansas, USA.
Clin Exp Allergy. 2022 May;52(5):697-705. doi: 10.1111/cea.14089. Epub 2022 Feb 3.
The airway microbiota plays an important role in asthma pathophysiology. However, the relationship between the airway microbiota and asthma phenotypes is still poorly understood.
We aimed to characterize the airway microbiota in asthma patients and determine its correlation with airway inflammatory phenotypes and other phenotypic characteristics.
The microbial composition of induced sputum specimens collected from asthma patients was determined using 16S rDNA gene sequencing.
Patients with asthma had a higher abundance of bacterial taxa associated with Bacteroidetes, Fusobacteria and Proteobacteria and a reduced abundance of Firmicutes and Actinobacteria compared to healthy controls. This study classified the asthma-associated lung microbiota into three community types based on DMM models, which were defined as three pulmotypes (P1, P2 and P3). The lungs of patients with pulmotype 3 (P3) were dominated by Faecalibacterium and Bacteroides, while patients with pulmotype 1 (P1) had a greater abundance of Pasteurellaceae, Streptococcus and Rothia. P1 patients were older (p = .045) and had lower blood TGF levels (p = .028). P3 patients had fewer eosinophils (p = .016) and more neutrophils (p = .039) in induced sputa than P1 patients.
Differences in asthma-associated airway microbiota pulmotypes are associated with and might influence asthma, particularly inflammatory phenotypes.
气道微生物群在哮喘发病机制中起着重要作用。然而，气道微生物群与哮喘表型之间的关系仍知之甚少。
我们旨在描述哮喘患者的气道微生物群，并确定其与气道炎症表型和其他表型特征的相关性。
通过 16S rDNA 基因测序确定从哮喘患者诱导痰标本中采集的微生物组成。
与健康对照组相比，哮喘患者中与拟杆菌门、梭杆菌门和变形菌门相关的细菌分类群丰度更高，厚壁菌门和放线菌门丰度更低。本研究基于 DMM 模型将与哮喘相关的肺微生物群分为三种群落类型，定义为三种肺型（P1、P2 和 P3）。肺型 3（P3）患者的肺部以粪杆菌和拟杆菌为主，而肺型 1（P1）患者的巴斯德氏菌科、链球菌属和罗氏菌属丰度更高。P1 患者年龄更大（p=0.045），血液 TGF 水平更低（p=0.028）。与 P1 患者相比，P3 患者诱导痰中的嗜酸性粒细胞（p=0.016）更少，中性粒细胞（p=0.039）更多。
哮喘相关气道微生物群的肺型差异与哮喘有关，并可能影响哮喘，特别是炎症表型。
