Sadek Khadiga M, Mamdouh Wael, Habib Shaymaa I, El Deftar Mervat, Habib A Nour A
Biomaterials Department, Faculty of Dentistry, Cairo University, 11 El-Saraya St.-Manial, Cairo, 11562 Cairo, Egypt.
Department of Chemistry, School of Sciences and Engineering (SSE), The American University in Cairo, AUC Avenue, 11835 New Cairo, Egypt.
ACS Omega. 2021 Dec 8;6(50):34447-34459. doi: 10.1021/acsomega.1c04608. eCollection 2021 Dec 21.
Different scaffold biomaterials are being investigated as a solution for bone loss due to disease or trauma. The aim of this study is the fabrication, characterization, and in vitro biological evaluation of a novel polycaprolactone (PCL) nanoscaffold incorporating pomegranate peel extract (PG) for bone regeneration. Using electrospinning, three groups of scaffolds were prepared: the control group PCL and two groups of PCL with PG concentrations (11 and 18 weight %). The antioxidant activity and the total phenolic content (TPC) of the fabricated nanoscaffolds were evaluated, in addition to the porosity and degradation measurement. Cultured osteoblasts derived from rabbit bone marrow mesenchymal stem cells were used for the assessment of cell proliferation and attachment on the scaffold's surface. Scaffolds' characterization showed uniform nanofibers (NFs) with a fiber diameter range of 149-168 nm. Meanwhile, higher antioxidant activity and TPC of the PG groups were detected. Furthermore, total porosities of 59 and 62% were determined for the PCL-PG scaffolds. An increased degradation rate and significant improvement in cell proliferation and cell attachment were revealed for the PCL-PG fabricated scaffolds. Such incorporation of natural food waste, PG, in PCL NFs offered novel PCL-PG scaffolds as a promising candidate for bone regeneration applications.
不同的支架生物材料正在被研究，作为治疗因疾病或创伤导致的骨质流失的解决方案。本研究的目的是制备、表征并对一种新型的聚己内酯（PCL）纳米支架进行体外生物学评估，该支架含有石榴皮提取物（PG）用于骨再生。使用静电纺丝法制备了三组支架：对照组PCL以及两组含有不同PG浓度（11%和18%重量）的PCL组。除了评估孔隙率和降解情况外，还对制备的纳米支架的抗氧化活性和总酚含量（TPC）进行了评估。使用源自兔骨髓间充质干细胞的培养成骨细胞来评估细胞在支架表面的增殖和附着情况。支架表征显示出均匀的纳米纤维（NFs），纤维直径范围为149 - 168 nm。同时，检测到PG组具有更高的抗氧化活性和TPC。此外，PCL - PG支架的总孔隙率分别为59%和62%。对于制备的PCL - PG支架，其降解速率增加，细胞增殖和细胞附着有显著改善。在PCL纳米纤维中掺入天然食物废料PG，为骨再生应用提供了一种有前景的新型PCL - PG支架。
