Department of Thyroid and Neck Tumour, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
Provincial Clinical College, Fujian Medical University, Fuzhou, China.
Bioengineered. 2022 Jan;13(1):190-205. doi: 10.1080/21655979.2021.2012316.
The phosphoprotein phosphatase catalytic subunit (PPPCs) family has been shown to play an important role in the development and progression of various malignancies, but its expression patterns and biological functions in breast cancer (BC) remain unclear. Therefore, we aimed to investigate the clinical significance and biological functions of the PPPCs family to understand its possible significance in the diagnosis, prognosis and treatment of breast cancer. We comprehensively investigated the expression levels, diagnostic accuracy, prognostic outcomes, biological functions and effects on immune cell infiltration of the PPPCs family in breast cancer using online databases. Except for PPP1CB, PPP1CC, PPP5C and PPEF1, the mRNA expression levels of the PPPCs family in breast cancer tissues were significantly different from those in paracancerous tissues. The differentially expressed genes (DEGs) were associated with the clinicopathological parameters and prognosis of breast cancer. The DEGs were mainly associated with the WNT signaling pathway, antigen presentation and DNA repair. In addition, the DEGs significantly affected the infiltration of immune cells in breast cancer tissues. Among the PPPCs family, PPP1CA and PPP4C played a prominent role in the progression of breast cancer, and inhibition of PPP1CA and PPP4C expression by siRNA can significantly inhibit breast cancer cells proliferation and migration. In conclusion, the PPPCs family, especially PPP1CA and PPP4C, could be used as new biomarkers to improve diagnostic accuracy, predict prognosis and novel targets for the treatment of breast cancer.
磷酸蛋白磷酸酶催化亚基（PPPCs）家族在各种恶性肿瘤的发生和发展中起着重要作用，但它在乳腺癌（BC）中的表达模式和生物学功能尚不清楚。因此，我们旨在研究 PPPCs 家族的临床意义和生物学功能，以了解其在乳腺癌诊断、预后和治疗中的可能意义。我们使用在线数据库全面研究了 PPPCs 家族在乳腺癌中的表达水平、诊断准确性、预后结果、生物学功能和对免疫细胞浸润的影响。除 PPP1CB、PPP1CC、PPP5C 和 PPEF1 外，PPPCs 家族在乳腺癌组织中的 mRNA 表达水平与癌旁组织明显不同。差异表达基因（DEGs）与乳腺癌的临床病理参数和预后相关。DEGs 主要与 WNT 信号通路、抗原呈递和 DNA 修复有关。此外，DEGs 显著影响乳腺癌组织中免疫细胞的浸润。在 PPPCs 家族中，PPP1CA 和 PPP4C 在乳腺癌的进展中起着突出的作用，siRNA 抑制 PPP1CA 和 PPP4C 的表达能显著抑制乳腺癌细胞的增殖和迁移。总之，PPPCs 家族，特别是 PPP1CA 和 PPP4C，可以作为新的生物标志物来提高诊断准确性、预测预后和为乳腺癌治疗提供新的靶点。
