Gilligan P H, Gage P A, Welch D F, Muszynski M J, Wait K R
J Clin Microbiol. 1987 Jul;25(7):1258-61. doi: 10.1128/jcm.25.7.1258-1261.1987.
During a 1-year period, the prevalence of thymidine-dependent (TD) Staphylococcus aureus in patients at two geographically distinct cystic fibrosis (CF) centers was determined. Of 200 CF patients who had their respiratory secretions cultured, 95 harbored S. aureus, and 20 (21%) had TD S. aureus as their predominant staphylococcal isolate. All 20 TD S. aureus-positive patients had received trimethoprim-sulfamethoxazole for an average of 30.9 months. It was also observed that TD S. aureus exhibited aberrant colony morphologies or did not grow on media commonly used in CF centers for S. aureus isolation, suggesting that this organism could be missed by routine culture methods. In contrast, all 20 isolates had typical staphylococcal morphology on mannitol salt agar after 48 h of incubation. Mannitol salt agar is recommended for primary isolation of TD S. aureus.
在1年的时间里，对两个地理位置不同的囊性纤维化（CF）中心患者中胸腺嘧啶核苷依赖性（TD）金黄色葡萄球菌的流行情况进行了测定。在200名呼吸道分泌物进行培养的CF患者中，95人携带金黄色葡萄球菌，其中20人（21%）以TD金黄色葡萄球菌作为主要的葡萄球菌分离株。所有20例TD金黄色葡萄球菌阳性患者均接受过甲氧苄啶-磺胺甲恶唑治疗，平均治疗时间为30.9个月。还观察到，TD金黄色葡萄球菌表现出异常的菌落形态，或者在CF中心常用的用于分离金黄色葡萄球菌的培养基上不生长，这表明这种微生物可能会被常规培养方法遗漏。相比之下，所有20株分离株在甘露醇盐琼脂上孵育48小时后均具有典型的葡萄球菌形态。推荐使用甘露醇盐琼脂对TD金黄色葡萄球菌进行初次分离。
