具有抗菌和抗炎作用的硫酸软骨素锌，用于皮肤创伤愈合。
Chondroitin sulfate zinc with antibacterial properties and anti-inflammatory effects for skin wound healing.
机构信息
Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.
出版信息
Carbohydr Polym. 2022 Feb 15;278:118996. doi: 10.1016/j.carbpol.2021.118996. Epub 2021 Dec 11.
A chondroitin sulfate zinc (CSZn) complex was prepared by an ion-exchange method. The purified product was characterized by energy-dispersive X-ray spectroscopy, high-performance chromatography, elemental analysis, Fourier transform infrared spectroscopy, inductively coupled mass spectrometry, and nuclear magnetic resonance spectroscopy. The CSZn demonstrated antibacterial activity against Escherichia coli and Staphylococcus aureus and satisfied MTT cell viability (NIH3T3 fibroblasts) at ≤50 μg/mL. RT-PCR demonstrated significant promotion by CSZn of fibroblast growth factor beta (β-FGF), collagen III (COLIIIα1), vascular endothelial growth factor (VEGF) and reduction of cytokines IL-6, IL-1β & TNF-alpha. An in vivo rat full-thickness wound healing model demonstrated significant wound healing of CSZn relative to controls of saline treatment, zinc chloride treatment and chondroitin treatment. CSZn has demonstrated promising antibacterial and wound healing properties making it deserving of consideration for more advanced wound healing applications.
采用离子交换法制备硫酸软骨素锌（CSZn）复合物。通过能谱、高效液相色谱、元素分析、傅里叶变换红外光谱、电感耦合质谱和核磁共振波谱对纯化产物进行了表征。CSZn 对大肠杆菌和金黄色葡萄球菌具有抗菌活性，并且在 ≤50μg/mL 时满足 MTT 细胞活力（NIH3T3 成纤维细胞）。RT-PCR 表明 CSZn 显著促进成纤维细胞生长因子β（β-FGF）、III 型胶原蛋白（COLIIIα1）、血管内皮生长因子（VEGF）的产生，同时降低细胞因子 IL-6、IL-1β 和 TNF-α 的水平。体内大鼠全层创面愈合模型表明，CSZn 相对于生理盐水处理、氯化锌处理和软骨素处理的对照组具有显著的创面愈合作用。CSZn 具有良好的抗菌和创面愈合性能，有望应用于更先进的创面愈合领域。
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