Manoussakis M N, Gharavi A E, Drosos A A, Kitridou R C, Moutsopoulos H M
Clin Immunol Immunopathol. 1987 Sep;44(3):297-307. doi: 10.1016/0090-1229(87)90073-0.
Quantitative determination of IgG and IgM antibodies to cardiolipin (anti-CL) was performed with a newly developed sensitive and specific ELISA method. We studied a cohort of 361 unselected patients with various autoimmune rheumatic diseases (ARD), 69 patients with thromboembolic phenomena (TEP) unassociated with ARD, and 267 healthy blood donors (HBD). Anti-CL of at least one immunoglobulin class were found in 42 (11.6%) of the ARD patients, in 3 (4.3%) of the TEP patients (2 with myocardial infarction and 1 with pulmonary emboli), and in 6 (2.3%) of the HBD. In ARD patients anti-CL were more prevalent in patients with systemic lupus erythematosus (SLE) and overlap syndromes. Significant correlations included CNS involvement (particularly seizures) and features of immune hyperreactivity (splenomegaly-lymphadenopathy, ANA, and antibodies to Ro(SSA), U1-nRNP, and double-stranded DNA). No statistical correlation could be demonstrated between the presence of anti-CL and thrombotic events, hematologic disorders, or recurrent abortions in the ARD patients.
采用新开发的灵敏且特异的酶联免疫吸附测定（ELISA）方法对心磷脂IgG和IgM抗体（抗心磷脂抗体）进行定量测定。我们研究了一组361例未经挑选的患有各种自身免疫性风湿性疾病（ARD）的患者、69例与ARD无关的血栓栓塞现象（TEP）患者以及267名健康献血者（HBD）。在42例（11.6%）ARD患者、3例（4.3%）TEP患者（2例心肌梗死和1例肺栓塞）以及6例（2.3%）HBD中发现至少一种免疫球蛋白类别的抗心磷脂抗体。在ARD患者中，抗心磷脂抗体在系统性红斑狼疮（SLE）患者和重叠综合征患者中更为普遍。显著相关性包括中枢神经系统受累（尤其是癫痫发作）以及免疫反应亢进特征（脾肿大-淋巴结病、抗核抗体以及抗Ro(SSA)、U1-nRNP和双链DNA抗体）。在ARD患者中，抗心磷脂抗体的存在与血栓形成事件、血液系统疾病或反复流产之间未显示出统计学相关性。
