Rennick D, Yang G, Muller-Sieburg C, Smith C, Arai N, Takabe Y, Gemmell L
DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304.
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6889-93. doi: 10.1073/pnas.84.19.6889.
Our studies show that although interleukin 4 (IL-4) fails to stimulate significant colony formation by bone marrow progenitor cells, it enhances erythroid, granulocyte, macrophage, and mast-cell colony formation when used as a costimulant with erythropoietin, granulocyte colony-stimulating factor, macrophage colony-stimulating factor, and interleukin 3 (IL-3), respectively. In contrast, IL-4 suppresses IL-3-dependent colony formation by granulocyte and macrophage progenitor cells and by multipotential progenitor cells. Furthermore, it appears to inhibit the in vitro generation of colony-forming progenitor cells from immature IL-3-dependent stem cells. We also found that IL-4 inhibits stromal cell-dependent growth of bone marrow-derived pre-B cells. The ability of IL-4 to directly or indirectly regulate both positive and negative aspects of progenitor cell growth is discussed.
我们的研究表明，虽然白细胞介素4（IL-4）不能刺激骨髓祖细胞形成显著的集落，但当它分别与促红细胞生成素、粒细胞集落刺激因子、巨噬细胞集落刺激因子和白细胞介素3（IL-3）共同刺激时，可增强红系、粒细胞、巨噬细胞和肥大细胞集落的形成。相比之下，IL-4抑制粒细胞和巨噬细胞祖细胞以及多能祖细胞依赖IL-3的集落形成。此外，它似乎还能抑制未成熟的依赖IL-3的干细胞在体外生成集落形成祖细胞。我们还发现，IL-4抑制骨髓来源的前B细胞依赖基质细胞的生长。文中讨论了IL-4直接或间接调节祖细胞生长的正负两个方面的能力。
