学龄前儿童肠道微生物群及其代谢产物的发育。
Development of gut microbiota along with its metabolites of preschool children.
机构信息
Department of Pediatrics, the First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, Xishan District, Kunming, 650032, China.
Department of Statistics, School of Public Health, Kunming Medical University, Kunming, 650000, China.
出版信息
BMC Pediatr. 2022 Jan 6;22(1):25. doi: 10.1186/s12887-021-03099-9.
BACKGROUND
To reveal the changes of intestinal microbial abundance and composition, as well as the microbiota metabolic levels of bile acids and short chain fatty acids of healthy preschool children during their growth.
METHODS
Feces of 120 healthy newborns and 150 healthy children aged 6 months to 6 years were collected. Then the composition of intestinal flora was analyzed by 16S rRNA, and the contents of bile acids and short chain fatty acids in feces were detected by LC-MS and GS methods, respectively.
RESULTS
The composition and function of intestinal microflora were not stable in neonatal period but significantly improved at 6 months after birth, and gradually stabilized and tended to adult-like formation after 2-3 years old. The levels of short chain fatty acids and secondary bile acids were consistent with the development of gut microbiota.
CONCLUSION
The age of 6 months may be a critical period for the development of intestinal microflora in children.
背景
揭示健康学龄前儿童在生长过程中肠道微生物丰度和组成的变化，以及胆汁酸和短链脂肪酸的微生物代谢水平。
方法
收集 120 名健康新生儿和 150 名 6 个月至 6 岁健康儿童的粪便。然后通过 16S rRNA 分析肠道菌群组成，通过 LC-MS 和 GS 方法分别检测粪便中胆汁酸和短链脂肪酸的含量。
结果
新生儿期肠道微生物组成和功能不稳定，但出生后 6 个月显著改善，2-3 岁后逐渐稳定并趋于成人形成。短链脂肪酸和次级胆汁酸的水平与肠道微生物群的发展一致。
结论
6 个月龄可能是儿童肠道微生物群发育的关键时期。
Zotero 插件