Jiang Pengfei, Chen Xiaowen, Qian Long, Ai Tao, Xu Qinyu, Xiang Wei, Hu Bolin, Liu Xiaochen, Wang Jun, Wang Chenghui
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs/National Demonstration Center for Experimental Fisheries Science Education/Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
Fisheries Technology Extension Station, Xinjiang Production and Construction Corps, Urumqi, Xinjiang 830002, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2022 Mar;41:100957. doi: 10.1016/j.cbd.2021.100957. Epub 2022 Jan 4.
Artificial domestication during aquaculture practice has strongly shaped the physiological characteristics of Perca fluviatilis. Thus, revealing the genetic changes in domesticated P. fluviatilis will improve aquaculture and selective breeding. In this study, comparative analysis of the liver transcriptome, proteome, and physiological and biochemical indices of domesticated and wild P. fluviatilis was conducted. Our results indicated that the activity of lipase and the content of glucose were higher; however, the total antioxidant capacity and superoxide dismutase were lower in domesticated P. fluviatilis. Integrated analysis of "omics" data identified 174 and 127 genes and proteins that showed consistent upregulation and downregulation in domesticated P. fluviatilis, respectively. GO and KEGG enrichment of differentially expressed genes and proteins and the protein-protein interaction network indicated that energy metabolism (lipid and carbohydrate metabolism) was enhanced, and that signal transduction and the stress response were reduced in domesticated P. fluviatilis. This study revealed that artificial domestication may significantly shape the physiological changes in energy metabolism and stress resistance in domesticated P. fluviatilis, which makes them more adaptable to the artificial aquaculture environment, thereby promoting growth and development.
水产养殖实践中的人工驯化极大地塑造了河鲈的生理特征。因此，揭示驯化河鲈的基因变化将有助于改善水产养殖和选育工作。本研究对驯化河鲈和野生河鲈的肝脏转录组、蛋白质组以及生理生化指标进行了比较分析。我们的结果表明，驯化河鲈的脂肪酶活性和葡萄糖含量较高；然而，其总抗氧化能力和超氧化物歧化酶活性较低。对“组学”数据的综合分析确定了174个和127个基因及蛋白质，它们在驯化河鲈中分别呈现出一致的上调和下调。差异表达基因和蛋白质的GO和KEGG富集分析以及蛋白质-蛋白质相互作用网络表明，驯化河鲈的能量代谢（脂质和碳水化合物代谢）增强，而信号转导和应激反应减弱。本研究表明，人工驯化可能显著塑造了驯化河鲈能量代谢和抗逆性的生理变化，使其更能适应人工养殖环境，从而促进生长发育。
