Hirose W, Kawagoe M, Hara M, Kitani A, Hirose T, Norioka K, Harigai M, Nakamura H
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Clin Exp Immunol. 1987 Oct;70(1):102-9.
Since thyroid follicular epithelial cells (thyrocytes) have been shown to express a number of functions similar to monocytes, they were further examined for their potency in secreting thymocyte-stimulating activity (TSA). Although spontaneous production of TSA could not be detected when thyrocytes were cultured in the culture medium, TSA was demonstrated in the culture supernatants after stimulation with the immune adjuvant lentinan. The release of TSA was found in the culture supernatants collected 24 h after stimulation and was maintained for the 4 days of culture. Maximum levels of TSA release were achieved by 2 days. In addition, when culture supernatants of thyrocytes stimulated with lentinan or monocyte-derived interleukin 1 (IL-1) were incubated with a rabbit antibody to human IL-1, a parallel reduction in TSA was observed, suggesting that the active product in the thyrocyte culture supernatant shared a common antigenic site with IL-1. The demonstration of the production of IL-1 like activity by thyrocytes provides additional evidence that these cells, in addition to their functions as endocrine cells, may also participate in the local immune responses under appropriate conditions.
由于甲状腺滤泡上皮细胞（甲状腺细胞）已被证明具有许多与单核细胞相似的功能，因此进一步检测了它们分泌胸腺细胞刺激活性（TSA）的能力。虽然在培养基中培养甲状腺细胞时未检测到TSA的自发产生，但在用免疫佐剂香菇多糖刺激后，在培养上清液中证实了TSA的存在。TSA的释放出现在刺激后24小时收集的培养上清液中，并在培养的4天内持续存在。TSA释放的最高水平在2天时达到。此外，当用香菇多糖或单核细胞衍生的白细胞介素1（IL-1）刺激的甲状腺细胞培养上清液与抗人IL-1兔抗体孵育时，观察到TSA平行降低，这表明甲状腺细胞培养上清液中的活性产物与IL-1具有共同的抗原位点。甲状腺细胞产生IL-1样活性的证明提供了额外的证据，表明这些细胞除了作为内分泌细胞发挥功能外，在适当条件下也可能参与局部免疫反应。
