Yang Zi-Feng, Wang Yu-Tao, Qin Sheng, Zhao Sui-Shan, Zhao Yun-Shi, Lin Qin, Guan Wen-Da, Huang Qun-Di, Mo Zi-Yao, Li Chu-Yuan, Zhong Nan-Shan
Clinical Virology Division, State Key Laboratory of Respiratory Diseases, Guangzhou Medical University, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
Bing Du Xue Bao. 2011 May;27(3):218-23.
This study was to investigate the antiviral effects of a hot water soluble extract S-03 isolated from Isatis indigotica root on different subtypes of influenza A and B viruses in MDCK cell cultures, using plaque reduction, immunofluorescence and hemo-agglutination inhibition (HAD) assays. Chemical analysis of the extract S-03 showed that it contained high proportion of polysaccharides. The antiviral effects in vitro showed that the S-03 had no effect on different influenza viruses if the drug was used before virus adsorption, but S-03 showed obvious activities against influenza viruses if treatment after virus adsorption or direct reaction of drug and virus before virus adsorption. Hemagglutination inhibition assay showed that S-03 inhibited HA activities of different human influenza viruses (inhibition concentration ranged from 3.12 to 25 mg/mL), avain influenza viruses (inhibition concentration ranged from 25 to 50 mg/mL). The antiviral effects of S-03 on different influenza A and B viruses in vitro might be through the inhibition of the HA to prevent infection.
本研究旨在利用蚀斑减少试验、免疫荧光试验和血凝抑制试验，研究从板蓝根中分离得到的水溶性提取物S-03在MDCK细胞培养物中对甲型和乙型流感病毒不同亚型的抗病毒作用。提取物S-03的化学分析表明，其含有高比例的多糖。体外抗病毒作用表明，如果在病毒吸附前使用该药物，S-03对不同流感病毒无作用，但如果在病毒吸附后进行处理或在病毒吸附前使药物与病毒直接反应，S-03对流感病毒显示出明显活性。血凝抑制试验表明，S-03抑制不同人类流感病毒的血凝素活性（抑制浓度范围为3.12至25mg/mL）、禽流感病毒的血凝素活性（抑制浓度范围为25至50mg/mL）。S-03在体外对不同甲型和乙型流感病毒的抗病毒作用可能是通过抑制血凝素以防止感染实现的。
