Aguirre Garcia Mayra, Hillion Killian, Cappelier Jean-Michel, Neunlist Michel, Mahe Maxime M, Haddad Nabila
UMR SECALIM, INRAE, Oniris, 44307 Nantes, France.
UMR Inserm 1235-TENS, INSERM, Université de Nantes, Institut des Maladies de l'Appareil Digestif-CHU de Nantes, 44035 Nantes, France.
Foods. 2022 Jan 1;11(1):108. doi: 10.3390/foods11010108.
Foodborne diseases cause high morbidity and mortality worldwide. Understanding the relationships between bacteria and epithelial cells throughout the infection process is essential to setting up preventive and therapeutic solutions. The extensive study of their pathophysiology has mostly been performed on transformed cell cultures that do not fully mirror the complex cell populations, the in vivo architectures, and the genetic profiles of native tissues. Following advances in primary cell culture techniques, organoids have been developed. Such technological breakthroughs have opened a new path in the study of microbial infectious diseases, and thus opened onto new strategies to control foodborne hazards. This review sheds new light on cellular messages from the host-foodborne pathogen crosstalk during in vitro organoid infection by the foodborne pathogenic bacteria with the highest health burden. Finally, future perspectives and current challenges are discussed to provide a better understanding of the potential applications of organoids in the investigation of foodborne infectious diseases.
食源性疾病在全球范围内导致高发病率和死亡率。了解细菌与上皮细胞在整个感染过程中的关系对于制定预防和治疗方案至关重要。对其病理生理学的广泛研究大多是在转化细胞培养物上进行的，这些培养物不能完全反映复杂的细胞群体、体内结构和天然组织的基因谱。随着原代细胞培养技术的进步，类器官得以发展。这些技术突破为微生物传染病的研究开辟了一条新途径，从而开启了控制食源性危害的新策略。本综述揭示了在体外类器官感染过程中，宿主与食源性病原体相互作用产生的细胞信号，这些食源性病原体对健康负担最大。最后，讨论了未来的前景和当前的挑战，以便更好地理解类器官在食源性传染病研究中的潜在应用。
