State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Arch Virol. 2022 Feb;167(2):619-623. doi: 10.1007/s00705-021-05328-z. Epub 2022 Jan 11.
Streptobotrys caulophylli is a pathogenic fungus that causes leaf and stem blight in many plants. We report a novel fusagravirus identified in S. caulophylli strain STB-2, provisionally named "Streptobotrys caulophylli fusagravirus 1" (ScFV1). The full-length genome of ScFV1 is 9270 nucleotides (nt) long and putatively possesses two large open reading frames (ORF1 and ORF2), which are separated by an intergenic region of 955 nt. A conserved domain search revealed that ORF2 (1051 aa) encodes a putative RNA-dependent RNA polymerase (RdRp), whereas ORF1 (1404 aa) encodes a protein of unknown function. Homology searches and phylogenetic analysis of the putative RdRp suggest that ScFV1 is a new member of the proposed family "Fusagraviridae". This is the first report of a mycovirus infecting the fungus S. caulophylli.
卷柏生棒束孢是一种病原菌，可引起许多植物的叶片和茎部枯萎病。我们报道了一种在卷柏生棒束孢 STB-2 菌株中鉴定出的新型呼肠孤病毒，暂命名为“卷柏生棒束孢呼肠孤病毒 1”（ScFV1）。ScFV1 的全基因组长 9270 个核苷酸（nt），推测具有两个大的开放阅读框（ORF1 和 ORF2），它们由 955 个核苷酸的基因间隔区隔开。保守结构域搜索表明，ORF2（1051 个氨基酸）编码一个推定的 RNA 依赖性 RNA 聚合酶（RdRp），而 ORF1（1404 个氨基酸）编码一个功能未知的蛋白。推定 RdRp 的同源搜索和系统发育分析表明，ScFV1 是拟议的“呼肠孤病毒科”的一个新成员。这是首例感染卷柏生棒束孢真菌的真菌病毒报告。
