Dizon Gian Vincent, Clarin Maria Thea Rane, Venault Antoine, Tayo Lemmuel, Chiang Heng-Chieh, Zheng Jie, Aimar Pierre, Chang Yung
R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan.
School of Chemical, Biological, and Materials Engineering and Sciences, Mapúa University, Manila 1002, Philippines.
ACS Appl Bio Mater. 2019 Jan 22;2(1):39-48. doi: 10.1021/acsabm.8b00212. Epub 2018 Dec 20.
Polydimethylsiloxane (PDMS) is extensively used in the field of biomaterials. However, its hydrophobicity still limits its range of applications and makes it prone to biofouling. Various techniques are currently utilized to overcome this limitation, but most of them reduce some of the PDMS prime characteristics, such as its mechanical strength and optical transparency. In this work, we employed an original two-step coating process to bypass harsh treatments on PDMS like UV-ozone or plasma treatment. A pre-coating step of tannic acid-Fe(III) complex was performed prior to the zwitterionization of the PDMS with poly(glycidyl methacrylate--sulfobetaine methacrylate) or poly(GMA--SBMA) by a "grafting-to" approach. Successful coating was evidenced by a decrease of the water-contact angle from 118° to 79°. The process was optimized, and the optimized coating condition led to a significant improvement of the PDMS biocompatibility while maintaining its mechanical property and optical transparency. In addition, a 90% reduction of attachment and fibrinogen plasma protein, an 80% reduction of red blood cells and cells from whole-blood attachment, and a 60% reduction of platelets adhesion were measured. We further tested the potential of the zwitterionic PDMS material as a storage vessel for platelet-rich plasma under physiological conditions. Platelet activation was decreased from 10.95% using virgin PDMS to 3.35% with the coated sample in a period of 2 days.
聚二甲基硅氧烷（PDMS）在生物材料领域有着广泛应用。然而，其疏水性仍限制了它的应用范围，使其易于发生生物污染。目前采用了各种技术来克服这一限制，但大多数技术都会降低PDMS的一些主要特性，如机械强度和光学透明度。在这项工作中，我们采用了一种独创的两步涂层工艺，以避免对PDMS进行如紫外线臭氧或等离子体处理等苛刻处理。在用聚（甲基丙烯酸缩水甘油酯 - 甲基丙烯酸磺基甜菜碱）或聚（GMA - SBMA）通过“接枝到”方法对PDMS进行两性离子化之前，先进行单宁酸 - 铁（III）络合物的预涂层步骤。水接触角从118°降至79°证明涂层成功。该工艺得到了优化，优化后的涂层条件在保持PDMS机械性能和光学透明度的同时，显著提高了其生物相容性。此外，还测得附着的纤维蛋白原血浆蛋白减少了90%，全血中红细胞和细胞的附着减少了80%，血小板黏附减少了60%。我们进一步测试了两性离子PDMS材料在生理条件下作为富血小板血浆储存容器的潜力。在2天的时间内，血小板活化率从使用未处理的PDMS时的10.95%降至使用涂层样品时的3.35%。
