Shi Yuting, Xia Wenjun, Liu Shima, Guo Jingyang, Qi Zhengnan, Zou Yan, Wang Liping, Duan Sheng-Zhong, Zhou Yi, Lin Chenglie, Shi Jiye, Wang Lihua, Fan Chunhai, Lv Min, Tang Zisheng
National Clinical Research Center of Oral Diseases, Shanghai 200011, China.
Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China.
ACS Appl Bio Mater. 2019 Jan 22;2(1):226-235. doi: 10.1021/acsabm.8b00566. Epub 2018 Dec 19.
Graphene-based nanomaterials (GMs) are served as great promising agents for the prevention and therapy of infectious diseases. However, their dental applications remain to be evaluated, especially under the context of the oral microbial community. Here, we examined the exposure-response of salivary bacterial community to two types of GMs, that is, graphene oxide (GO) and GO-silver nanoparticles (AgNPs). Both GO and GO-AgNPs showed lethal effect against salivary bacteria in a concentration-dependent manner, and the antibacterial capacity of GO-AgNPs is superior to GO. Interestingly, the salivary bacterial community enhanced the tolerance to GMs as compared to homogeneous bacteria. High-throughput sequencing revealed that both 80 μg/mL GO and 20 μg/mL GO-AgNPs significantly altered the biodiversity of salivary bacterial community. Especially, they increased the relative abundance of Gram-positive bacteria compared to the untreated sample, notably , suggesting that the bacterial wall structure plays a critical role in resisting the damage of GMs. Although GMs could effectively limit the salivary bacterial activity and cause changes in bacterial community structure, they are not toxic to mammalian cell lines. We envision this study could provide novel insights into the application of GMs as "green antibiotics" in nanomedicine.
基于石墨烯的纳米材料（GMs）有望成为预防和治疗传染病的理想药物。然而，它们在牙科领域的应用仍有待评估，尤其是在口腔微生物群落的背景下。在此，我们研究了唾液细菌群落对两种GMs，即氧化石墨烯（GO）和氧化石墨烯-银纳米颗粒（GO-AgNPs）的暴露反应。GO和GO-AgNPs均对唾液细菌表现出浓度依赖性的致死作用，且GO-AgNPs的抗菌能力优于GO。有趣的是，与单一细菌相比，唾液细菌群落对GMs的耐受性增强。高通量测序显示，80 μg/mL的GO和20 μg/mL的GO-AgNPs均显著改变了唾液细菌群落的生物多样性。特别是，与未处理样本相比，它们增加了革兰氏阳性菌的相对丰度，这表明细菌细胞壁结构在抵抗GMs的损伤中起关键作用。尽管GMs可以有效限制唾液细菌的活性并导致细菌群落结构的变化，但它们对哺乳动物细胞系无毒。我们设想这项研究可以为GMs作为纳米医学中的“绿色抗生素”的应用提供新的见解。
