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使用扫描电子显微镜分析一种草药阿育吠陀药用漱口水对牙本质小管的封闭作用。

Analysis of an Herbal Ayurdantham Medicated Tooth liquid on Dentinal Tubule Occlusion using Scanning Electron Microscopy.

作者信息

Shivkumar Ashwini Tumkur, Kalgeri Sowmya Halasabalu, Avinash Bhagyalakshmi, Shashidar Harsha Raj Bassapa, Sheshadri Ramya

机构信息

Department of Conservative Dentistry and Endodontics, JSS Dental College and Hospital, JSSAHER, Mysore, Karnataka, India.

Department of Orthodontics and Dentofacial Orthopedics, JSS Dental College and Hospital, JSSAHER, Mysore, Karnataka, India.

出版信息

J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1184-S1187. doi: 10.4103/jpbs.jpbs_275_21. Epub 2021 Nov 10.

Abstract

BACKGROUND

Dentin hypersensitivity is described as "short sharp pain arising from exposed dentin in response to various stimuli typically thermal, evaporative, tactile, osmotic or chemical and cannot be ascribed to any other form of dental defect or disease." Dentine hypersensitivity can be treated with various desensitizing agents ,where oral rinse has a promising effect in delivering the therapeutic effect all over the tooth surface including interproximal areas, as they can flow and reach areas where toothpastes are not much effective, hence the occlusion of dentinal tubules using Ayurdhantam medicated an herbal mouthwash was evaluated.

METHODOLOGY

Twenty such dentinal blocks were obtained; the surfaces of each dentin block were polished using a silicon carbide disc for 30 s to create a basic smear layer. The smear layer is finally detached by applying 17% EDTA solution onto the dentin blocks for 5 min to ensure the patency of the dentinal tubules, following which they were rinsed with distilled water. The specimens were placed into the Ayurdhantam solution (medicated) for 2 min every day for 7 days. The specimens were stored in artificial saliva during the study. The specimens were analyzed under scanning electron microscope (SEM) for the occlusion of dentinal tubules.

RESULTS

SEM images showed the partial occlusion of the tubules and a large amount of smear layer.

CONCLUSIONS

Based on this experiment carried out, it is shown that Ayurdhantam-medicated mouthwash can be used for treating dentine hypersensitivity effectively.

摘要

背景

牙本质过敏被描述为“因暴露的牙本质对各种刺激(通常为热、蒸发、触觉、渗透或化学刺激)产生的短暂尖锐疼痛,且不能归因于任何其他形式的牙齿缺陷或疾病”。牙本质过敏可用多种脱敏剂治疗,口腔含漱液在使治疗效果覆盖整个牙齿表面(包括邻面区域)方面具有显著效果,因为它们能够流动并到达牙膏效果不佳的区域,因此评估了使用Ayurdhantam药用草本漱口水封闭牙本质小管的效果。

方法

获取20个这样的牙本质块;使用碳化硅盘对每个牙本质块的表面进行30秒的抛光,以形成基本的玷污层。通过在牙本质块上涂抹17%的乙二胺四乙酸(EDTA)溶液5分钟来最终去除玷污层,以确保牙本质小管通畅,之后用蒸馏水冲洗。将标本每天置于Ayurdhantam溶液(含药)中2分钟,持续7天。在研究过程中,将标本保存在人工唾液中。在扫描电子显微镜(SEM)下分析标本牙本质小管的封闭情况。

结果

扫描电子显微镜图像显示小管部分封闭且有大量玷污层。

结论

基于所进行的这项实验,结果表明Ayurdhantam药用漱口水可有效用于治疗牙本质过敏。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fad/8686859/74fed14ec295/JPBS-13-1184-g001.jpg

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