Heping Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi, China.
Department of Biochemistry, Changzhi Medical College, Changzhi, China.
Medicine (Baltimore). 2023 Oct 13;102(41):e35461. doi: 10.1097/MD.0000000000035461.
Gene polymorphisms of estrogen receptor (ESR) 1 PvuII (rs2234693), XbaI (rs9340799), G2014A (rs2228480), ESR2 AluI (rs4986938), and RsaI (rs1256049) had been reported to be associated with the risk of osteoporosis. However, these conclusions were inconsistent, therefore, an updated meta-analysis was conducted to further explore these issues.
To evaluate the association between gene polymorphisms of ESR1 PvuII (rs2234693), XbaI (rs9340799), G2014A (rs2228480), ESR2 AluI (rs4986938), RsaI (rs1256049), and osteoporosis risk.
PubMed, Medline, Ovid, Embase, CNKI, and China Wanfang databases were searched. Association was assessed using odds ratio with 95% confidence interval. Moreover, the false-positive reporting probability, Bayesian false-finding probability, and Venetian criteria were used to assess the credibility of statistically significant associations.
Overall, ESR1 PvuII (rs2234693) and XbaI (rs9340799) were associated with the risk of osteoporosis in Indians. Moreover, ESR1 G2014A (rs2228480) was associated with the decreased risk of osteoporosis in East Asians. Moreover, ESR2 Alul (rs4986938) was associated with the increased risk of osteoporosis in East Asians and Caucasians. There was a significant association between ESR2 Rsal (rs1256049) and osteoporosis risk in overall population. When only high-quality and Hardy-Weinberg equilibrium studies were included in the sensitivity analysis, all results did not change in the present study. When the credibility was evaluated applying false-positive reporting probability, Bayesian false-finding probability, and Venetian criteria, all significant associations were considered as false positive results.
In summary, this study shows that all substantial associations between gene polymorphisms of ESR1 (PvuII, XbaI, and G2014A) and ESR 2 (AluI and RsaI) and osteoporosis risk are possibly false positive results instead of real associations or biological variables.
雌激素受体（ESR）1 PvuII（rs2234693）、XbaI（rs9340799）、G2014A（rs2228480）、ESR2 AluI（rs4986938）和 RsaI（rs1256049）的基因多态性与骨质疏松症的风险有关。然而，这些结论并不一致，因此，进行了一项更新的荟萃分析以进一步探讨这些问题。
评估 ESR1 PvuII（rs2234693）、XbaI（rs9340799）、G2014A（rs2228480）、ESR2 AluI（rs4986938）、RsaI（rs1256049）基因多态性与骨质疏松症风险之间的关联。
检索 PubMed、Medline、Ovid、Embase、CNKI 和中国万方数据库。使用优势比和 95%置信区间评估相关性。此外，还使用虚假阳性报告概率、贝叶斯假发现概率和威尼斯标准来评估统计学显著关联的可信度。
总体而言，ESR1 PvuII（rs2234693）和 XbaI（rs9340799）与印度人骨质疏松症的风险相关。此外，ESR1 G2014A（rs2228480）与东亚人骨质疏松症风险降低相关。此外，ESR2 AluI（rs4986938）与东亚人和高加索人骨质疏松症风险增加相关。ESR2 Rsal（rs1256049）与总体人群骨质疏松症风险之间存在显著关联。当仅包括高质量和 Hardy-Weinberg 平衡研究的敏感性分析时，本研究中的所有结果均未发生变化。当应用虚假阳性报告概率、贝叶斯假发现概率和威尼斯标准评估可信度时，所有显著关联均被认为是假阳性结果。
总之，本研究表明，ESR1（PvuII、XbaI 和 G2014A）和 ESR2（AluI 和 RsaI）基因多态性与骨质疏松症风险之间的所有实质性关联可能都是假阳性结果，而不是真实关联或生物学变量。
