Ren Pengfei, Wang Faming, Bernaerts Katrien V, Fu Yifu, Hu Wanjun, Zhou Naizhen, Dai Jidong, Liang Min, Zhang Tianzhu
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Aachen-Maastricht Institute for Biobased Materials (AMIBM), Faculty of Science and Engineering, Maastricht University, Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD Geleen, The Netherlands.
ACS Appl Bio Mater. 2020 Oct 19;3(10):6768-6778. doi: 10.1021/acsabm.0c00711. Epub 2020 Sep 15.
In recent decades, in vitro three-dimensional (3D) cell culture has been rapidly developed and widely used in many biomedical fields. Based on this background, a kind of self-assembled supramolecular hybrid hydrogel materials based on host-guest interaction of β-cyclodextrin (βCD) and adamantane (Ad) is designed for 3D cell culture. First, βCD is grafted to poly(methyl vinyl ether--maleic acid) (PMM) to obtain the host polymers of βCD-grafted-PMM (PMM-βCD). Second, the guest polymers of poly(acrylamide---adamantyl acrylamide) (PAAm-Ad) are synthesized through free-radical copolymerization of acrylamide and -adamantyl acrylamide. Finally, the self-assembled supramolecular hybrid hydrogels of PMM-βCD/PAAm-Ad are formed by simply mixing the aqueous solution of host and guest polymers with a total concentration of 3.3% (w/v) and a βCD/Ad molar ratio of 1:1. The main cross-linking interactions come from the host-guest interaction of βCD/Ad as well as hydrogen-bonding interaction of carboxyl/amide groups. The prepared hydrogels with good cytocompatibility have been successfully used as 3D cell culture scaffold for SKOV3, HUVEC, and L929 cells culture. Thus, this work provides a way and biomaterial for the preparation of a functionalized 3D cell culture scaffold, which lays an experimental and theoretical basis for cell follow-up research.
近几十年来，体外三维（3D）细胞培养迅速发展并广泛应用于许多生物医学领域。基于此背景，设计了一种基于β-环糊精（βCD）与金刚烷（Ad）主客体相互作用的自组装超分子杂化水凝胶材料用于3D细胞培养。首先，将βCD接枝到聚（甲基乙烯基醚-马来酸）（PMM）上，得到接枝βCD的PMM（PMM-βCD）主体聚合物。其次，通过丙烯酰胺与金刚烷基丙烯酰胺的自由基共聚合成聚（丙烯酰胺-金刚烷基丙烯酰胺）（PAAm-Ad）客体聚合物。最后，将主体和客体聚合物的水溶液以总浓度3.3%（w/v）和βCD/Ad摩尔比1:1简单混合，形成PMM-βCD/PAAm-Ad自组装超分子杂化水凝胶。主要的交联相互作用来自βCD/Ad的主客体相互作用以及羧基/酰胺基团的氢键相互作用。所制备的具有良好细胞相容性的水凝胶已成功用作SKOV3、HUVEC和L929细胞培养的3D细胞培养支架。因此，这项工作为制备功能化3D细胞培养支架提供了一种方法和生物材料，为细胞后续研究奠定了实验和理论基础。
