Godal T, Henriksen A, Iversen J G, Landaas T O, Lindmo T
Int J Cancer. 1978 May 15;21(5):561-9. doi: 10.1002/ijc.2910210505.
Peripheral blood lymphocytes consisting mainly of neoplastic B cells from patients with chronic lymphocytic leukemia (CLL cells) showed a markedly reduced response to the human B-cell mitogens anti-beta2 microglobulin, Sepharose-bound protein A and Sepharose-bound anti-human immunoglobulin (anti F(ab')2) in all of nine patients studied. On the other hand, CLL cells from three out of eight patients tested responded well to the calcium ionophore A23187. Sepharose-bound protein A and anti-beta2 microglobulin also failed to induce increased uptake of 86Rubidium (potassium analogue) in CLL cells as compared to B-cell-enriched preparations of normal peripheral blood lymphocytes. The capacity of CLL cells to cap various surface markers including beta2 microglobulin was reduced. On the other hand, surface concentrations of beta2 microglobulin were not reduced as measured by fluorescein-labelled anti-beta2-microglobulin in single-cell cytofluorometry. It is concluded that various membrane-associated events elicited by ligand-receptor interactions are altered or blocked in CLL cells.
在研究的所有9例慢性淋巴细胞白血病患者中，主要由肿瘤性B细胞（CLL细胞）组成的外周血淋巴细胞对人B细胞有丝分裂原抗β2微球蛋白、琼脂糖结合蛋白A和琼脂糖结合抗人免疫球蛋白（抗F(ab')2）的反应明显降低。另一方面，在检测的8例患者中，有3例患者的CLL细胞对钙离子载体A23187反应良好。与正常外周血淋巴细胞的B细胞富集制剂相比，琼脂糖结合蛋白A和抗β2微球蛋白也未能诱导CLL细胞对86铷（钾类似物）摄取增加。CLL细胞覆盖包括β2微球蛋白在内的各种表面标志物的能力降低。另一方面，通过单细胞细胞荧光测定法用荧光素标记的抗β2微球蛋白测量，β2微球蛋白的表面浓度并未降低。得出的结论是，配体-受体相互作用引发的各种膜相关事件在CLL细胞中发生改变或受阻。
