Jones E H, Hockley A B, Lawler S D
Br J Cancer. 1981 Nov;44(5):675-81. doi: 10.1038/bjc.1981.253.
Cells from patients with chronic lymphocytic leukaemia (CLL) do not respond in the mixed lymphocyte culture (MLC) but are able to stimulate the response of normal lymphocytes. Mixed lymphocyte cultures were performed using cells from 24 patients with CLL and cells from 16 normal donors. The stimulatory capacity of 8 of these CLL cells was reduced when a common DR antigen was shared with the normal responding cell. We suggest that cells from certain selected CLL patients may be used in the mixed-lymphocyte reaction for determining the D-locus specificity of normal donors. CLL cells when expressed 1 DR antigen only, induced more clearly defined typing responses than cells with 2 DR antigens. There was no correlation between the ability of a CLL cell to induce a typing response and the T-cell status of the patient. However, a correlation with clinical course was suggested, because most cells which induced a typing response were obtained from patients who had received intensive treatment for the disease.
慢性淋巴细胞白血病（CLL）患者的细胞在混合淋巴细胞培养（MLC）中无反应，但能够刺激正常淋巴细胞的反应。使用24例CLL患者的细胞和16名正常供体的细胞进行混合淋巴细胞培养。当这些CLL细胞中的8个与正常反应细胞共享一种常见的DR抗原时，其刺激能力降低。我们建议，某些选定的CLL患者的细胞可用于混合淋巴细胞反应，以确定正常供体的D位点特异性。仅表达1种DR抗原的CLL细胞比表达2种DR抗原的细胞诱导出更明确的分型反应。CLL细胞诱导分型反应的能力与患者的T细胞状态之间没有相关性。然而，提示与临床病程存在相关性，因为大多数诱导分型反应的细胞来自接受过该疾病强化治疗的患者。
