sp. nov., isolated from oil-contaminated soil.

A yellow-coloured, Gram-stain-positive, motile, aerobic and rod-shaped bacteria, designated DKR-3, was isolated from oil-contaminated experimental soil. Strain DKR-3 could grow at pH 5.0-10.5 (optimum, pH 7.0-8.5), at 10-40 °C (optimum, 25-32 °C) and tolerated 3.5 % of NaCl. Phylogenetic analyses based on its 16S rRNA gene sequence indicated that strain DKR-3 formed a lineage within the family and was clustered with members of the genus . Strain DKR-3 had highest 16S rRNA gene sequence similarities to DSM 20111 (98.3 %), JCM 18111 (98.2 %) and DSM 20107 (97.8 %). The predominant respiratory quinone was tetrahydrogenated menaquinone with nine isoprene units [MK-9(H)]. The principal cellular fatty acids were anteiso-C, C and anteiso-C. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell-wall diamino acid was l-ornithine whereas rhamnose and glucose were the cell-wall sugars. The DNA G+C content was 74.2mol %. The genome of strain DKR-3 was 3.74 Mb and contained three putative biosynthetic gene clusters. The average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain DKR-3 and its phylogenetically related members were below the species threshold values. Based on a polyphasic study, strain DKR-3 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is DKR-3 (=KACC 22071=NBRC 114730).